FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002135077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002135077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1989368
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66210.3328192672245658No Hit
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACCGTCCATTATCTCGTA56330.2831552533266847TruSeq Adapter, Index 22 (97% over 36bp)
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT37460.18830100815937525No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26740.1344145477357633No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26640.1339118755303192No Hit
TGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTT23270.11697182220685162No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)