FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002135081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002135081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1603132
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACAGCCGTAAATCTCGTA97770.6098686820548775TruSeq Adapter, Index 13 (97% over 35bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77020.480434549369609No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33170.20690747861061973No Hit
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT31600.19711414905322833No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30930.1929348300701377No Hit
TGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTT20000.12475579054001791No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)