FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002135086

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002135086
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1948357
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115270.5916266885380862No Hit
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACGAGTAGAGATCTCGTA48290.24784985503170107TruSeq Adapter, Index 9 (97% over 35bp)
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT42930.2203394963038088No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34400.17655901870139817No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33010.16942480253875444No Hit
TGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTT26860.1378597454162661No Hit
GTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACT19550.10034095394221901No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)