FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002135087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002135087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2104022
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138100.6563619581924524No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA57760.27452184435333854No Hit
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACGAGTAGAGATCTCGTA54210.25764939720212054TruSeq Adapter, Index 9 (97% over 35bp)
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT48110.2286573049141121No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37310.17732704315829398No Hit
TGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTT29810.14168102805008695No Hit
GTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACT21700.10313580371307904No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)