FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002135090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002135090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1798172
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155990.8674920975301583No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55840.31053759039735906No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52660.29285296401011696No Hit
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACACGTCGTTATCTCGTA40450.2249506721270268TruSeq Adapter, Index 5 (97% over 35bp)
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT36570.20337320345328477No Hit
TGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTT24390.13563774766818748No Hit
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18120.1007690031876817No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)