FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002135125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002135125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1334141
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70380.527530448430863No Hit
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACAGCTAGTGATCTCGTA41480.3109116652587695TruSeq Adapter, Index 13 (97% over 35bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40310.3021419774971311No Hit
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT30530.2288363823613846No Hit
TGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTT24020.18004094020047356No Hit
TGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAG16160.1211266275453644No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14990.11235693978372602No Hit
TGTTTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCC13960.10463661636963409No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)