FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002135127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002135127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1422939
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76200.5355113606416017No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50740.3565859112723736No Hit
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACAGCTAGTGATCTCGTA41020.2882765881039173TruSeq Adapter, Index 13 (97% over 35bp)
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT33480.23528766869134937No Hit
TGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTT26690.18756953038745863No Hit
TGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAG16510.11602746147234702No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15480.10878892208309704No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)