FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002135136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002135136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4314187
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACGCGTATCAATCTCGTA406300.9417765154825231TruSeq Adapter, Index 6 (97% over 35bp)
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT80390.18633870066364763No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70580.16359976978281193No Hit
GTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAG53950.1250525301754421No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44360.10282354473739781No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)