FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002135141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002135141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences351602
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12280.349258536640861No Hit
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACCGTCCATTATCTCGTA10340.2940825137513438TruSeq Adapter, Index 22 (97% over 36bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8460.24061296579655406No Hit
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT6160.17519809329867292No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4760.13538034482170183No Hit
TGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTT3610.10267290857276123No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)