FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002135150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002135150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454089
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29310.6454681791454979No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17320.3814230250017067No Hit
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACGAGTAGAGATCTCGTA11360.2501712219410732TruSeq Adapter, Index 9 (97% over 35bp)
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT9850.21691782888376507No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7850.1728735996687874No Hit
TGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTT5910.13015069733025905No Hit
GTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACT4630.10196239063267334No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)