FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002135151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002135151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences484464
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28830.5950906568909146No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20800.42934046699032336No Hit
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACGAGTAGAGATCTCGTA11950.24666435483338287TruSeq Adapter, Index 9 (97% over 35bp)
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT10030.2070329271111992No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8070.16657584464480332No Hit
TGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTT6010.12405462531787709No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)