FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002135153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002135153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1223005
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110220.9012228077563053No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA66530.543987964072101No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34400.281274401985274No Hit
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACACGTCGTTATCTCGTA28510.2331143372267489TruSeq Adapter, Index 5 (97% over 35bp)
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT24060.19672854976063056No Hit
TGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTT14940.12215812690872074No Hit
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12840.10498730585729411No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)