FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002135156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002135156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1282502
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124200.9684195424256649No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA78360.6109931992308784No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35880.27976564558963646No Hit
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACACGTCGTTATCTCGTA29600.23079886035265443TruSeq Adapter, Index 5 (97% over 35bp)
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT25170.19625700388771322No Hit
TGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTT16090.12545789402277735No Hit
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13810.1076801439685864No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)