FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002135203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002135203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2753808
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96260.34955232899316147No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA95250.34588468041344933No Hit
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACACTCTCCAATCTCGTA45690.1659157065416325TruSeq Adapter, Index 8 (97% over 36bp)
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39130.14209414744964063No Hit
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT33370.121177656539599No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)