FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002135207

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002135207
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2852683
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97270.3409772484359461No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA83050.29112943849702194No Hit
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACCGTCCATTATCTCGTA60990.2137987291262296TruSeq Adapter, Index 22 (97% over 36bp)
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT48640.1705061515773046No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31180.10930061279153695No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)