FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002135208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002135208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2801370
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98050.35000731784805295No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA75260.2686542655914784No Hit
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACCGTCCATTATCTCGTA62960.22474717727397667TruSeq Adapter, Index 22 (97% over 36bp)
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT47090.16809632429846824No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32410.11569339287562871No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)