FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002135210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002135210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2133363
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACAGCCGTAAATCTCGTA124300.5826481475491981TruSeq Adapter, Index 13 (97% over 35bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104250.48866507950123816No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA72110.3380109245355807No Hit
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT37010.17348196251645875No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34830.16326335461897482No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)