FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002135212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002135212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2253495
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACAGCCGTAAATCTCGTA127000.5635690338784866TruSeq Adapter, Index 13 (97% over 35bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110980.4924794596837357No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA85600.37985440393699565No Hit
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT40270.17870019680540672No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34920.15495929655934448No Hit
TGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTT22730.1008655444099055No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)