FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002135214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002135214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2683070
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167380.623837618847067No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA91210.33994640467822307No Hit
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACGAGTAGAGATCTCGTA63780.23771276932767313TruSeq Adapter, Index 9 (97% over 35bp)
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT52920.19723674745720388No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40020.14915749495913264No Hit
TGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTT28560.1064452287864275No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)