FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002135215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002135215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2891647
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179060.6192318771966288No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA125700.43470036280362023No Hit
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACGAGTAGAGATCTCGTA63810.22067008870723157TruSeq Adapter, Index 9 (97% over 35bp)
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT59590.20607632951048313No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40830.14119980758370576No Hit
TGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTT34540.11944749825964233No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)