FastQCFastQC Report
Fri 9 Sep 2022
EGAF00002135218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002135218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2460466
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226710.9214108221775876No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA125810.511325903304496No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63500.2580811927496661No Hit
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACACGTCGTTATCTCGTA50160.20386382091847643TruSeq Adapter, Index 5 (97% over 35bp)
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT44270.1799252661894129No Hit
TGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTT26680.1084347436623794No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)