FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002135264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002135264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1996217
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACGCGTATCAATCTCGTA195180.9777494130147174TruSeq Adapter, Index 6 (97% over 35bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34870.17468040799171633No Hit
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT33470.1676671423998493No Hit
GTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAG26210.13129835083059607No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20620.10329538321735562No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20350.10194282485320985No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)