FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002135268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002135268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2651695
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102110.3850744523785729No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA94170.3551313405199316No Hit
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACACTCTCCAATCTCGTA78100.29452859397479725TruSeq Adapter, Index 8 (97% over 36bp)
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43170.16280152883344426No Hit
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT30590.11536017528411073No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)