FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002135270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002135270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2689938
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97060.3608261603055535No Hit
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACCGTCCATTATCTCGTA82540.3068472210140159TruSeq Adapter, Index 22 (97% over 36bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA75740.281567827957373No Hit
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT45230.16814513940470005No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35250.13104391253627407No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)