FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002135278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002135278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2678929
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181940.679152004401759No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA112840.4212131041920111No Hit
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACGAGTAGAGATCTCGTA70930.26476998830502785TruSeq Adapter, Index 9 (97% over 35bp)
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT52150.1946673465403525No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43230.1613704581196441No Hit
TGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTT30380.11340352805169528No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)