FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002135279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002135279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2760696
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189570.686674664649784No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA121980.44184509993132165No Hit
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACGAGTAGAGATCTCGTA77140.2794222906107735TruSeq Adapter, Index 9 (97% over 35bp)
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT53650.19433505174057555No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45690.16550174303871198No Hit
TGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTT31020.1123629693381669No Hit

[OK]Adapter Content

Adapter graph