FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002135317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002135317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2209994
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118990.5384177513604109No Hit
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACAGCTAGTGATCTCGTA69380.3139375039027255TruSeq Adapter, Index 13 (97% over 35bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA64580.29221798792213916No Hit
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT48640.22009109526994192No Hit
TGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTT35850.16221763498000447No Hit
TGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAG26780.12117679957502148No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25500.11538492864686511No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)