FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002135318

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002135318
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2144112
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114430.5336941353809876No Hit
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACAGCTAGTGATCTCGTA68460.3192930220063131TruSeq Adapter, Index 13 (97% over 35bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA59650.2782037505503444No Hit
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT45780.21351496563612346No Hit
TGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTT35610.16608274194631625No Hit
TGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAG24960.11641182923280127No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23890.1114214182841195No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)