FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002135327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002135327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1763476
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACGCGTATCAATCTCGTA177451.0062512900657565TruSeq Adapter, Index 6 (97% over 35bp)
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT30480.17284045827672168No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28410.16110227754729864No Hit
GTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAG23530.1334296582431516No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17690.10031324497753302No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)