FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002135331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002135331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2383522
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83120.3487276391826885No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA76360.32036624793058344No Hit
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACACTCTCCAATCTCGTA73360.30777983169444206TruSeq Adapter, Index 8 (97% over 36bp)
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39240.1646303243687283No Hit
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT29040.12183650916584784No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)