FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002135332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002135332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2364343
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87960.3720272397025305No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA76850.32503744169098986No Hit
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACACTCTCCAATCTCGTA70960.3001256585867617TruSeq Adapter, Index 8 (97% over 36bp)
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39010.16499298113683167No Hit
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT28600.1209638364653521No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)