FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002135345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002135345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2209206
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206540.9349060250605874No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA126760.5737808063168396No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63160.2858945702664215No Hit
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACACGTCGTTATCTCGTA52300.23673663750686896TruSeq Adapter, Index 5 (97% over 35bp)
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT40850.18490806199150284No Hit
TGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTT24070.10895317141090509No Hit
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22440.10157495498382677No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)