FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002146287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002146287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422916
Sequences flagged as poor quality0
Sequence length20-100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144363.4134438044434354No Hit
ATGGGCAGAAATCACATTGCGTCAGCATCCGCGAGGACCATCGCAATGCT51051.207095498869752No Hit
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18500.43743911320451345No Hit
ATGGGGCGACCCCAGGTCAGACGGGACCACCCGCTGAATTTAAGCATATA10910.2579708500033103No Hit
ATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8750.20689687786699962No Hit
ATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8140.19247320980998592No Hit
ACTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAA8080.19105448836175506No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC8080.19105448836175506No Hit
ATGGGGGGGCTTTCCCCGAGCATGAAACAGTCGACTCAGAACTGGTACGG7960.18821704546529333No Hit
GCATGAAACAGTCGACTCAGAACTGGTACGGACAAGGGGAATCCGACTGT7230.17095593451181795No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6770.1600790700753814No Hit
TTGTTCGCTATCGGTCTCTCGCCTGTATTTAGCCTTGGACGGAGTCTACC6660.15747808075362485No Hit
ATGTAGGCAAGGGAAGTCGGCAAAACGGATCCGTAACTTCGGGAAAAGGA6400.15133028781129113No Hit
AAATCACATTGCGTCAGCATCCGCGAGGACCATCGCAATGCTTTGTTTTA6310.14920220563894485No Hit
ATGGGGGGGGCTTTCCCCGAGCATGAAACAGTCGACTCAGAACTGGTACG6030.14258150554720087No Hit
GCGTCAGCATCCGCGAGGACCATCGCAATGCTTTGTTTTAATTAAACAGT5730.13548789830604657No Hit
TTCCCAAACAACCCGACTCGTTGACAGCGCCTCGTGATGCGACAGGGTCC5550.13123173396135404No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA5430.1283942910648923No Hit
CCCCAGGTCAGACGGGACCACCCGCTGAATTTAAGCATATAAATAAGCGG5210.12319231242137918No Hit
ATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5100.12059132309962262No Hit
GGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGAT5080.12011841595021235No Hit
GCCTTGGACGGAGTCTACCGCCCGATTTGGGCTGCATTCCCAAACAACCC4720.11160608726082721No Hit
GTATTTAGCCTTGGACGGAGTCTACCGCCCGATTTGGGCTGCATTCCCAA4670.11042381938730149No Hit
CCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTTCATGCTCGGGGAAAGC4650.10995091223789122No Hit
TCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCA4630.10947800508848092No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG4550.1075863764908398No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT4480.10593120146790379No Hit
CGCGAGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCT4440.10498538716908323No Hit
GTCGTACACATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGCCAA4420.10451248001967293No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC4400.10403957287026266No Hit
ATGGGGGGCTTTCCCCGAGCATGAAACAGTCGACTCAGAACTGGTACGGA4370.10333021214614722No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)