FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002146288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002146288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428716
Sequences flagged as poor quality0
Sequence length20-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185204.319876095130576No Hit
ATGGGCAGAAATCACATTGCGTCAGCATCCGCGAGGACCATCGCAATGCT58291.3596413476520586No Hit
ATGGGGCGACCCCAGGTCAGACGGGACCACCCGCTGAATTTAAGCATATA16350.3813713507310201No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC9970.23255488481885445No Hit
ATGGGGGGGCTTTCCCCGAGCATGAAACAGTCGACTCAGAACTGGTACGG9100.2122617303762864No Hit
ACTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAA9050.21109545713246064No Hit
ATGTAGGCAAGGGAAGTCGGCAAAACGGATCCGTAACTTCGGGAAAAGGA8820.2057306002108622No Hit
TTGTTCGCTATCGGTCTCTCGCCTGTATTTAGCCTTGGACGGAGTCTACC8510.19849970609914255No Hit
GCATGAAACAGTCGACTCAGAACTGGTACGGACAAGGGGAATCCGACTGT7540.17587400516892301No Hit
CCCCAGGTCAGACGGGACCACCCGCTGAATTTAAGCATATAAATAAGCGG7230.16864311105720337No Hit
AAATCACATTGCGTCAGCATCCGCGAGGACCATCGCAATGCTTTGTTTTA6790.15837990651153677No Hit
ATGGGGGGGGCTTTCCCCGAGCATGAAACAGTCGACTCAGAACTGGTACG6690.15604736002388528No Hit
TTCCCAAACAACCCGACTCGTTGACAGCGCCTCGTGATGCGACAGGGTCC6590.15371481353623379No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA6420.14974948450722622No Hit
GCGTCAGCATCCGCGAGGACCATCGCAATGCTTTGTTTTAATTAAACAGT6340.14788344731710504No Hit
CCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTTCATGCTCGGGGAAAGC6220.14508439153192323No Hit
GTCGTACACATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGCCAA5990.13971953461032477No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT5640.13155562190354456No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTT5630.1313223672547794No Hit
ATGGGTGATCATTGGTCCGATGCTTCGGGCGAAACCAATTCCCAGGGTGT5620.13108911260601425No Hit
GCCTTGGACGGAGTCTACCGCCCGATTTGGGCTGCATTCCCAAACAACCC5450.12712378357700668No Hit
GTTCTGAGTCGACTGTTTCATGCTCGGGGAAAGCCCCCGAAGGGACGATT5430.1266572742794764No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG5410.1261907649819461No Hit
GTATTTAGCCTTGGACGGAGTCTACCGCCCGATTTGGGCTGCATTCCCAA5390.12572425568441578No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT5320.12409147314305974No Hit
ACCGAGTACCGTCCACGCCCGGTCGTACACATAACCGCATCAGGTCTCCA5300.12362496384552946No Hit
GGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGAT5280.12315845454799915No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTT5200.12129241735787794No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTT5170.12059265341158248No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC5140.11989288946528703No Hit
TGCCTACATTGTTCCATTGGCCAGAGGCTGTTCACCTTGGAGACCTGATG5030.1173270883288704No Hit
ATGGGGGGCTTTCCCCGAGCATGAAACAGTCGACTCAGAACTGGTACGGA4990.1163940697338098No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTT4930.11499454184121889No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA4910.1145280325436886No Hit
TCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCA4890.1140615232461583No Hit
CGCGAGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCT4880.11382826859739316No Hit
GAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATT4820.11242874070480226No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTT4710.1098629395683856No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTT4630.10799690237826438No Hit
GACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCG4630.10799690237826438No Hit
GGTGTGGGTCGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTC4570.1065973744856735No Hit
GTCCGGGCCGGACGGGGCTCTCACCCTCCCAGGCGCCCCTTTCCAGGGGA4500.10496459194431745No Hit
GTCCACGCCCGGTCGTACACATAACCGCATCAGGTCTCCAAGGTGAACAG4460.10403157334925685No Hit
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4340.10123251756407506No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)