FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002146289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002146289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428716
Sequences flagged as poor quality0
Sequence length20-100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132943.1008873006839024No Hit
ATGGGCAGAAATCACATTGCGTCAGCATCCGCGAGGACCATCGCAATGCT48491.1310517918622118No Hit
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20670.4821373589975648No Hit
ATGGGGCGACCCCAGGTCAGACGGGACCACCCGCTGAATTTAAGCATATA11070.2582128961830209No Hit
ATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8520.1987329607479077No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC8440.1968669235577865No Hit
ATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8220.19173532128495321No Hit
ACTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAA8010.18683697366088506No Hit
ATGGGGGGGCTTTCCCCGAGCATGAAACAGTCGACTCAGAACTGGTACGG7680.17913957025163513No Hit
TTGTTCGCTATCGGTCTCTCGCCTGTATTTAGCCTTGGACGGAGTCTACC7620.17774004235904423No Hit
GCATGAAACAGTCGACTCAGAACTGGTACGGACAAGGGGAATCCGACTGT6700.15628061467265045No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6650.1551143414288247No Hit
ATGGGGGGGGCTTTCCCCGAGCATGAAACAGTCGACTCAGAACTGGTACG6060.14135231715168084No Hit
ATGTAGGCAAGGGAAGTCGGCAAAACGGATCCGTAACTTCGGGAAAAGGA5720.13342165909366574No Hit
AAATCACATTGCGTCAGCATCCGCGAGGACCATCGCAATGCTTTGTTTTA5710.1331884044449006No Hit
GCGTCAGCATCCGCGAGGACCATCGCAATGCTTTGTTTTAATTAAACAGT5580.13015609401095365No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA5250.1224586906017037No Hit
CCCCAGGTCAGACGGGACCACCCGCTGAATTTAAGCATATAAATAAGCGG4990.1163940697338098No Hit
ATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4920.11476128719245375No Hit
CCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTTCATGCTCGGGGAAAGC4820.11242874070480226No Hit
TTCCCAAACAACCCGACTCGTTGACAGCGCCTCGTGATGCGACAGGGTCC4810.11219548605603709No Hit
GGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGAT4810.11219548605603709No Hit
ATGGGGGGCTTTCCCCGAGCATGAAACAGTCGACTCAGAACTGGTACGGA4650.1084634116757947No Hit
GCCTTGGACGGAGTCTACCGCCCGATTTGGGCTGCATTCCCAAACAACCC4630.10799690237826438No Hit
GTATTTAGCCTTGGACGGAGTCTACCGCCCGATTTGGGCTGCATTCCCAA4550.1061308651881432No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT4470.104264827998022No Hit
TCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCA4290.1000662443202493No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)