FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002146402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002146402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381017
Sequences flagged as poor quality0
Sequence length20-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98412.5828243884131155No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC29340.7700443812218353No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT16730.4390880196946593No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT14640.3842348241679505No Hit
AATCAGGACCGGTCATCAGGCTTGCGCCCTAGTCCGATCCCTATGTTTTA12890.338305114994869No Hit
ATGGGCAGAAATCAGGACCGGTCATCAGGCTTGCGCCCTAGTCCGATCCC12580.3301689950842088No Hit
GTTCTGGGACGACCACACAGCCCCCGGGCCGGCTCAGCCCTCCCCATTGC11850.3110097449720091No Hit
ATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGC11520.3023487140993709No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA10870.2852891078350835No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC10220.26822950157079606No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC9330.24487096376277173No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT8390.22020014854980224No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA8300.2178380492209009No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA7760.20366545324749288No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG7710.20235317584254772No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACA7150.18765566890716162No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT7050.18503111409727124No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC6900.1810942818824357No Hit
TCCCAGAACTGGCCGCGATCAATGGGAATCCAACTGTCTAATTAAAACAT6810.17873218255353435No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC6610.1734830729337536No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGC6500.17059606264287422No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC6390.1677090523519948No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT6310.16560940850408248No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAA6250.16403467561814827No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA6030.15826065503638945No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT6010.15773574407441138No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG5980.15694837763144426No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG5930.15563610022649907No Hit
TTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTT5710.14986207964474027No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG5620.1474999803158389No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAATCGTGCGCCGGACGGCCGAAG5190.1362143946333103No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCG5130.1346396617473761No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA4990.13096528501352958No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT4980.13070282953254053No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGAGCTCGAT4880.12807827472265015No Hit
CCCTGGGAGCAGGCGGCCACTAGCCGGGCAACCGGCCGGCGGCTGCCAGC4830.12676599731770496No Hit
ATGGGGTCCTGCGATGCTGGGGCCTCCCAGGGAGAGCGCCTCCTCTTTTG4780.12545371991275978No Hit
GAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT4700.1233540760648475No Hit
TCTCCGGGAGGTGTGGCGGGAACTGCTTCTGGGTGGATCCCTTCGCTGAG4610.12099197673594617No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCC4560.11967969933100098No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTAC4550.11941724385001193No Hit
GTTCTAAGTTGGTTGTTAATCGTGCGCCGGACGGCCGAAGCCTGCCAGAG4510.11836742192605579No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGT4410.11574286711616544No Hit
ATGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGG4370.11469304519220927No Hit
CCCTAGTCCGATCCCTATGTTTTAATTAGACAGTTGGATTCCCATTGATC4370.11469304519220927No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG4360.11443058971122025No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCC4270.1120684903823189No Hit
CTCCCAGGGCGTCCTTCAAAGGCCCAACGCACCCAACCCTTAGAGCCAAT4200.11023130201539563No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTC4160.10918148009143949No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGC4040.10603201431957104No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCA3980.10445728143363682No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGAT3940.10340745950968068No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA3930.10314500402869163No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAG3910.10262009306671355No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)