FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002146555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002146555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326722
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT9910.30331596892771223No Hit
GTTCTAAGTTGGTTGTTAAACGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGTCTGCACCCCAGCGCTGAAAGG9300.2846456620613243No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTG9080.27791210876524997No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCAATATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATT7710.23598043596696885No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC7230.2212890469573521No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTT6670.20414909311279925No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGTCTGCA5870.17966344476343804No Hit
ATCCTAAGAGATAGGGTAATTCCGTTTTAATGTAGGCGCTTGCGCCTCGCCCTCGAAAGGGAAGCCGGTTAAAAT5450.16680847938002338No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAAC5420.16589026756692232No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGTCTGCACCCCAG5140.1573202906446459No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTGCGTTG5060.1548717258097098No Hit
TCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCG5050.15456565520534277No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT5010.1533413727878747No Hit
GTCTGTCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTCAATGGCTGGAAGAGCGCTGCACTTTTGCGGCGTTT4770.14599567828306634No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGA4670.1429349722393962No Hit
GTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCGCCACCGGGTGCTGGAAGG4530.13864998377825796No Hit
CTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAA4360.13344678350401873No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTAC4310.13191643048218363No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAAAACTATTTCTTCAAGCGG4260.13038607746034855No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTACGCCGGACGGCCG4260.13038607746034855No Hit
CATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATGA4100.1254889477904763No Hit
ACTTATACCCCTCCGCTGGGGCAAAATTTACGCCCCAGCGAGTAGGCAGGCGTGGAGGTCCGTGACGAAGCCTTG4030.12334645355990718No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCA3980.12181610053807213No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAG3740.11447040603326376No Hit
GTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCAACCTGCTCCCAGGGCTTCTGCTCAAGGCCCAACGCACCCAA3680.11263398240706166No Hit
ATCCTAAGCCATAGGGAAACTCCGTTTTAAAGTGCGCTCTCGGGCGCCGCCTGGCGAAAGGGAAACCGGTTAATA3660.11202184119832763No Hit
ACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAA3620.11079755878085956No Hit
CCTTTGTGCTGTCCGGTGCGCTCTCGACGACCCTTGAAAATCCGCCGGAAGGAATGATTTTCACGCGAGGTCGTA3570.10926720575902449No Hit
CTTCTTAACAGTCTGTCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTCAATGGCTGGAAGAGCGCTGCACTTT3570.10926720575902449No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTA3420.10467614669351925No Hit
GGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCTCTAGTAACG3420.10467614669351925No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)