FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002146558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002146558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences439026
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTAAGTTGGTTGTTAAACGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGTCTGCACCCCAGCGCTGAAAGG20180.4596538701580316No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTG18140.413187373868518No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT16010.36467088509564355No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGA14540.33118767453408227No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAAAACTATTTCTTCAAGCGG14500.33027656676369965No Hit
ATCCTAAGAGATAGGGTAATTCCGTTTTAATGTAGGCGCTTGCGCCTCGCCCTCGAAAGGGAAGCCGGTTAAAAT14470.32959323593591267No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTT12330.28084897022044253No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGTCTGCA11030.2512379676830074No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGTCTGCACCCCAG10910.2485046443718595No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAAC10760.24508799023292469No Hit
ACTTATACCCCTCCGCTGGGGCAAAATTTACGCCCCAGCGAGTAGGCAGGCGTGGAGGTCCGTGACGAAGCCTTG10640.24235466692177685No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTACGCCGGACGGCCG9770.22253807291595487No Hit
GTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAAAACTATTTC9720.22139918820297658No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC9670.2202603034899983No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT9500.21638809546587218No Hit
GAGTACGACCTGGCGTGAAAACTATTTCTTCAAGCGGATTTTCAAGGGTCGTCGGGAGCGCACCAAACGCCGCAA9100.207277017762046No Hit
CTTCCAGCCATTGAACCCTAGCTCCGGACAAACCGATTTCAGGGTGACAGACTGTTAAGAAGAAAAGAGAACTCT8720.19862149394341108No Hit
GTTCTAAGTTGGTTGTTAAACGTGCGCCGGACGGCCGAAGCCTGCCAAGGGTGTCAGCACCCCAGCGCTGAAAGG8530.19429373203409367No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTACGCCGGACGGCCGAAGCCTGCCAAGGG8470.19292707037851972No Hit
CTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAA8380.19087707789515884No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTAC8350.19019374706737185No Hit
CCCTTATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGC8210.1870048698710327No Hit
TGCCAAGGGTGTCTGCACCCCAGCGCTGAAAGGCGCCCACCTTGCGGTTAGTGCCCTTCTGCGCCAGAGGCTTCT7780.17721046133941953No Hit
AGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTGGGCC7600.17311047637269775No Hit
CTTCTTAACAGTCTGTCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTCAATGGCTGGAAGAGCGCTGCACTTT7420.16901049140597596No Hit
TCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCG7260.16536606032444548No Hit
GTCTGTCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTCAATGGCTGGAAGAGCGCTGCACTTTTGCGGCGTTT7220.16445495255406284No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCA7200.16399939866887156No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTGCGTTG7150.16286051395589327No Hit
CCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAAAACTATTTCTTCAAGCGGATTTTCAAGGGTCGT7080.1612660753577237No Hit
GTTGGACACGGGTCAATCGATCCTAAGAGATAGGGTAATTCCGTTTTAATGTAGGCGCTTGCGCCTCGCCCTCGA6880.1567105365058106No Hit
CTTCTTGGGGCTCCCGCCAACGTCTCCGCTCTCAGTTGCGTTGCCGCGGAGAATCCACATCCAGATGCCGGAATT6790.1546605440224497No Hit
GGGTAATTCCGTTTTAATGTAGGCGCTTGCGCCTCGCCCTCGAAAGGGAAGCCGGTTAAAATTCCGGCATCTGGA6770.15420499013725839No Hit
GTTAAGAAGAAAAGAGAACTCTTCTTGGGGCTCCCGCCAACGTCTCCGCTCTCAGTTGCGTTGCCGCGGAGAATC6660.15169944376870617No Hit
GCCGAAGCCTGCCAAGGGTGTCTGCACCCCAGCGCTGAAAGGCGCCCACCTTGCGGTTAGTGCCCTTCTGCGCCA6460.14714390491679308No Hit
CTCTAAGGGTTGGGTACGTTGGGCCTTGGGGAGAAGCCTCTGGCGCAGAAGGGCACTAACCGCAAGGTGGGCGCC6430.14646057408900612No Hit
GTTTTAATGTAGGCGCTTGCGCCTCGCCCTCGAAAGGGAAGCCGGTTAAAATTCCGGCATCTGGATGTGGATTCT6330.14418280466304959No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTA6320.14395502772045393No Hit
GTGACAGACTGTTAAGAAGAAAAGAGAACTCTTCTTGGGGCTCCCGCCAACGTCTCCGCTCTCAGTTGCGTTGCC6280.1430439199500713No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAG6250.14236058912228433No Hit
GTCTGCACCCCAGCGCTGAAAGGCGCCCACCTTGCGGTTAGTGCCCTTCTGCGCCAGAGGCTTCTCCCCAAGGCC6220.14167725829449737No Hit
ACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAAAACT6190.1409939274667104No Hit
TTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTG6180.14076615052411476No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGTTACTTA5900.1343883961314364No Hit
GTTATGAGTACGACCTGGCGTGAAAACTATTTCTTCAAGCGGATTTTCAAGGGTCGTCGGGAGCGCACCAAACGC5850.13324951141845812No Hit
GTGTCCAACTGCTGTTCACACGGAACCTTTCCCCACTTCAGTCCTCAAAGTTCTCATTTGAGTATTTGCTACTAC5820.13256618059067116No Hit
GCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTACGCCGGAC5770.1314272958776929No Hit
CATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATGA5730.13051618810731028No Hit
GCTCTAAGGGTTGGGTACGTTGGGCCTTGGGGAGAAGCCTCTGGCGCAGAAGGGCACTAACCGCAAGGTGGGCGC5640.12846619562394937No Hit
TCTTAACAGTCTGTCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTCAATGGCTGGAAGAGCGCTGCACTTTTG5540.1261884261979928No Hit
ATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAAAACTATTTCTTCAA5530.12596064925539718No Hit
AAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGT5460.1243662106572276No Hit
GAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTA5390.122771772059058No Hit
CTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAA5390.122771772059058No Hit
CCCCAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCC5350.1218606642886754No Hit
ACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAA5270.12003844874791014No Hit
CCCTAGCTCCGGACAAACCGATTTCAGGGTGACAGACTGTTAAGAAGAAAAGAGAACTCTTCTTGGGGCTCCCGC5230.11912734097752752No Hit
GCCTTGGGGGTGACCCCGGGTCGAACGGCCTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAAC5200.11844401014974057No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTT5120.11662179460897532No Hit
GGTATAAGTAACACGCTTGAGCGCCATCCATTTTCAGGGCTAGTTCATTCGGCAGGTGAGTTGTTACACACTCCT5090.11593846378118837No Hit
CCCCCAAGGCTTCGTCACGGACCTCCACGCCTGCCTACTCGCTGGGGCGTAAATTTTGCCCCAGCGGAGGGGTAT5080.11571068683859269No Hit
CCCTTGGCAGGCTTCGGCCGTCCGGCGTACGTTTAACAACCAACTTAGAACTGGTACGGACAAGGGGAATCTGAC5070.11548290989599705No Hit
TAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTACGCCGGACGGCCGAAGC5020.11434402518301877No Hit
CTGTTAAGAAGAAAAGAGAACTCTTCTTGGGGCTCCCGCCAACGTCTCCGCTCTCAGTTGCGTTGCCGCGGAGAA4980.11343291741263614No Hit
GTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGTTACTTATACCCCTCCG4950.1127495865848492No Hit
CTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAACTTTGAGGACTGAAGTGGGGAAAGGTTCCG4910.11183847881446657No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTGCGCCGGACGGCCGAAGCCTGCCAAGGGTGTCAGCA4840.110244040216297No Hit
GCGCTGAAAGGCGCCCACCTTGCGGTTAGTGCCCTTCTGCGCCAGAGGCTTCTCCCCAAGGCCCAACGTACCCAA4750.10819404773293609No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTGCGCCGGACGGCCGAAGCCTGCCAAGGGTGTCAGCACCCCAG4740.10796627079034044No Hit
CCCAAGAAGAGTTCTCTTTTCTTCTTAACAGTCTGTCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTCAATGG4730.1077384938477448No Hit
GAAGGGCACTAACCGCAAGGTGGGCGCCTTTCAGCGCTGGGGTGCAGACACCCTTGGCAGGCTTCGGCCGTCCGG4710.10728293996255346No Hit
CTAAGAGATAGGGTAATTCCGTTTTAATGTAGGCGCTTGCGCCTCGCCCTCGAAAGGGAAGCCGGTTAAAATTCC4670.10637183219217085No Hit
ACCCCGGGTCGAACGGCCTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAACTTTGAGGACTGA4610.10500517053659691No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4560.10386628582361865No Hit
CTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTAGATGAACCAACACCTTTTGTGGTGTCTGAT4550.103638508881023No Hit
TCTTAGGATCGATTGACCCGTGTCCAACTGCTGTTCACACGGAACCTTTCCCCACTTCAGTCCTCAAAGTTCTCA4550.103638508881023No Hit
CTCTTAGGATCGATTGACCCGTGTCCAACTGCTGTTCACACGGAACCTTTCCCCACTTCAGTCCTCAAAGTTCTC4540.10341073193842734No Hit
ACCCGGGGTCACCCCCAAGGCTTCGTCACGGACCTCCACGCCTGCCTACTCGCTGGGGCGTAAATTTTGCCCCAG4510.10272740111064037No Hit
GTGCCCTTCTGCGCCAGAGGCTTCTCCCCAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGT4400.10022185474208817No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)