FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002146582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002146582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences272646
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23510.8622902958414941No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15500.5685027471519846No Hit
TCCGAAGACTGGCCAGGGGCGCCGACTCGAGTCCACAGCAGGTCCGCCGCAGCTTGCGCCACAGTAGTCCCGCCG8090.29672175641674553No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7250.265912575280767No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6220.22813465079260287No Hit
GGGTAGGGTACGTCGGGGCCTTGGGCAAAGACGAGGGACCGCGGCGGGACTACTGTGGCGCAAGCTGCGGCGGAC5670.20796197266785502No Hit
GTTCATAGTTGGTAGTTAATCGTACGCCAGACGTCCGAAGACTGGCCAGGGGCGCCGACTCGAGTCCACAGCAGG5100.18705574261129815No Hit
GTCCGTACCAGTTCATAGTTGGTAGTTAATCGTACGCCAGACGTCCGAAGACTGGCCAGGGGCGCCGACTCGAGT4920.18045377522501704No Hit
GAATAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCATAGGGTAGGGTA4050.14854426619132502No Hit
GGGTACGATCGCGTGTAAGACTTTTTCATTCCCTCGGATTTTCAAGGGCCGTTGAGGACGCACCGGACACCACAA3960.14524328249818447No Hit
ATCGTACCCATATCCGCATCAGGTCCCCAAGGTGATCAGCCTCTAGTCCATAGAATAATGTAGATAAGGGAAGTC3950.14487650653227996No Hit
CCCCTTGTCCGTACCAGTTCATAGTTGGTAGTTAATCGTACGCCAGACGTCCGAAGACTGGCCAGGGGCGCCGAC3800.13937486704371235No Hit
GTCCTAAGAGATAGGGAAACTCCGTTTTAAAGTATGCGCTTGTTCGCATCGCCTATCGAAAGGGAATACGGTTAA3580.13130579579381324No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT3550.13020546789609969No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3540.1298386919301952No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGGTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA3500.12837158806657717Illumina PCR Primer Index 11 (96% over 29bp)
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCATAGTTGGTAGTTAATCGTACGCCAGACGTCCG3230.11846863698715551No Hit
CCATAGAATAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCATAGGGTA3200.11736830908944199No Hit
GTCTTCGGACGTCTGGCGTACGATTAACTACCAACTATGAACTGGTACGGACAAGGGGAATCTGACTGTCTAATT3170.11626798119172847No Hit
ACATTATTCTATGGACTAGAGGCTGATCACCTTGGGGACCTGATGCGGATATGGGTACGATCGCGTGTAAGACTT3160.11590120522582396No Hit
GACTAGAGGCTGATCACCTTGGGGACCTGATGCGGATATGGGTACGATCGCGTGTAAGACTTTTTCATTCCCTCG3010.11039956573725637No Hit
GGGTACGTCGGGGCCTTGGGCAAAGACGAGGGACCGCGGCGGGACTACTGTGGCGCAAGCTGCGGCGGACCTGCT3000.11003278977135186No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2990.10966601380544737No Hit
GTAGAGCACCGCTATTTTGTGGTGTCCGGTGCGTCCTCAACGGCCCTTGAAAATCCGAGGGAATGAAAAAGTCTT2970.10893246187363835No Hit
ACTTATAACCCTGGAATCGGATTATCCGGAGATAGGGTCTAATGGTTGGTAGAGCACCGCTATTTTGTGGTGTCC2960.10856568590773383No Hit
GGCTCATAGGGTAGGGTACGTCGGGGCCTTGGGCAAAGACGAGGGACCGCGGCGGGACTACTGTGGCGCAAGCTG2900.1063650301123068No Hit
ATTCTATGGACTAGAGGCTGATCACCTTGGGGACCTGATGCGGATATGGGTACGATCGCGTGTAAGACTTTTTCA2830.10379759835097525No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2830.10379759835097525No Hit
CCTTGGGGACCTGATGCGGATATGGGTACGATCGCGTGTAAGACTTTTTCATTCCCTCGGATTTTCAAGGGCCGT2820.10343082238507076No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCA2760.1012301665896437No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)