FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002146583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002146583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences272646
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14320.525223183175253No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9050.33193224914357816No Hit
TCCGAAGACTGGCCAGGGGCGCCGACTCGAGTCCACAGCAGGTCCGCCGCAGCTTGCGCCACAGTAGTCCCGCCG7380.27068066283752557No Hit
GGGTAGGGTACGTCGGGGCCTTGGGCAAAGACGAGGGACCGCGGCGGGACTACTGTGGCGCAAGCTGCGGCGGAC5270.19329093403167477No Hit
GTCCGTACCAGTTCATAGTTGGTAGTTAATCGTACGCCAGACGTCCGAAGACTGGCCAGGGGCGCCGACTCGAGT4860.17825311942959002No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4520.16578273658883683No Hit
GTTCATAGTTGGTAGTTAATCGTACGCCAGACGTCCGAAGACTGGCCAGGGGCGCCGACTCGAGTCCACAGCAGG4270.15661333744122416No Hit
GGGTACGATCGCGTGTAAGACTTTTTCATTCCCTCGGATTTTCAAGGGCCGTTGAGGACGCACCGGACACCACAA3790.13900809107780784No Hit
CCCCTTGTCCGTACCAGTTCATAGTTGGTAGTTAATCGTACGCCAGACGTCCGAAGACTGGCCAGGGGCGCCGAC3790.13900809107780784No Hit
GAATAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCATAGGGTAGGGTA3700.1357071073846673No Hit
ATCGTACCCATATCCGCATCAGGTCCCCAAGGTGATCAGCCTCTAGTCCATAGAATAATGTAGATAAGGGAAGTC3530.1294719159642907No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT3520.1291051399983862No Hit
GTCCTAAGAGATAGGGAAACTCCGTTTTAAAGTATGCGCTTGTTCGCATCGCCTATCGAAAGGGAATACGGTTAA3400.12470382840753212No Hit
ACATTATTCTATGGACTAGAGGCTGATCACCTTGGGGACCTGATGCGGATATGGGTACGATCGCGTGTAAGACTT3370.1236035005098186No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3140.11516765329401493No Hit
CCATAGAATAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCATAGGGTA3050.11186666960087438No Hit
GTAGTTAATCGTACGCCAGACGTCCGAAGACTGGCCAGGGGCGCCGACTCGAGTCCACAGCAGGTCCGCCGCAGC2940.10783213397592482No Hit
ACCCTACCCTATGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTATTCT2890.10599825414640231No Hit
GGGTACGTCGGGGCCTTGGGCAAAGACGAGGGACCGCGGCGGGACTACTGTGGCGCAAGCTGCGGCGGACCTGCT2870.10526470221459328No Hit
GGCTCATAGGGTAGGGTACGTCGGGGCCTTGGGCAAAGACGAGGGACCGCGGCGGGACTACTGTGGCGCAAGCTG2860.10489792624868877No Hit
ACTTATAACCCTGGAATCGGATTATCCGGAGATAGGGTCTAATGGTTGGTAGAGCACCGCTATTTTGTGGTGTCC2850.10453115028278427No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAG2810.10306404641916625No Hit
GTCTTCGGACGTCTGGCGTACGATTAACTACCAACTATGAACTGGTACGGACAAGGGGAATCTGACTGTCTAATT2750.10086339062373921No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)