FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002146588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002146588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences391379
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTAGACCGTGCCAGTTCTGAATTGATTGTTAATTGATAACTCACACCCTTTAGGAAGGGCCGTTGCCAGCCC39741.0153840650622543No Hit
CCCTAGACCGTGCCAGTTCTGAATTGATTGTTAATTGATAACTCACACCCTTTAGGAAGGGCCGTTGCCAGCCCA26410.6747934866203859No Hit
ATGTAGGTAAGGGAAGTCGGCAAATTCGATCCGTAACTTCGGGATAAGGATTGGCTCTGAAGATTGAGATAACTC22450.573612789648908No Hit
CCCTTTAGGAAGGGCCGTTGCCAGCCCAACCAAAGTGTATAACAGGCTTAGCAAAACCGCAAAACTTGCGAATCG21670.5536832584272534No Hit
CTTTAGGAAGGGCCGTTGCCAGCCCAACCAAAGTGTATAACAGGCTTAGCAAAACCGCAAAACTTGCGAATCGCA20610.5265995365106457No Hit
GTGTATAACAGGCTTAGCAAAACCGCAAAACTTGCGAATCGCAGTTATATAGCCCAGCCCGAGTTATCTCAATCT17520.4476479320556289No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16900.4318065098025188No Hit
ATATAGCCCAGCCCGAGTTATCTCAATCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCGAATTTGCCGACT16510.4218417441916914No Hit
TATCTATACAATCTCGGTAACGAGAAACTTTCATGAAGAAGCCAATAGGAGGTATCGGAAGAGTTTTCTTTTCTG16420.41954218289688516No Hit
TACCTACATTGATCTATCGACTAGAGACTCTTAACCTTGGAGACCTGCTGCGGATTCGGTACAGACTGTTGAGAA15280.3904144064960052No Hit
GTTATATAGCCCAGCCCGAGTTATCTCAATCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCGAATTTGCCG13400.3423791261156066No Hit
GGGCTATATAACTGCGATTCGCAAGTTTTGCGGTTTTGCTAAGCCTGTTATACACTTTGGTTGGGCTGGCAACGG12390.3165729382516691No Hit
CAGTTATATAGCCCAGCCCGAGTTATCTCAATCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCGAATTTGC12000.3066081726408417No Hit
ATATAACTGCGATTCGCAAGTTTTGCGGTTTTGCTAAGCCTGTTATACACTTTGGTTGGGCTGGCAACGGCCCTT11110.28386806650331264No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10940.27952445072423404No Hit
GTGTCAACACCCGCGAGGGCCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAGACCGTGCCAGTTC10570.27007069873447476No Hit
GTATAAGGTATCTTGTATATCTATACAATCTCGGTAACGAGAAACTTTCATGAAGAAGCCAATAGGAGGTATCGG10550.2695596851134067No Hit
GTTATACACTTTGGTTGGGCTGGCAACGGCCCTTCCTAAAGGGTGTGAGTTATCAATTAACAATCAATTCAGAAC10320.2636830284711239No Hit
GCATTAAATTGCTGTGTCGATATTCTCTTATTGGACCTTGAAAATTCATGAAAGGGCCACGCAAATTCTCAACAG10200.26061694674471547No Hit
GTATAGGATACACGTTGATGTCTTCATTGTAGCGCGCGTGCAGCCCAGGACATCTAAGGGCATCACGGACCTGTT9610.24554204492320741No Hit
GTGTAATAAATTGGTAGAGCATGGCATTAAATTGCTGTGTCGATATTCTCTTATTGGACCTTGAAAATTCATGAA9430.24094292233359482No Hit
GGTTAAGAGTCTCTAGTCGATAGATCAATGTAGGTAAGGGAAGTCGGCAAATTCGATCCGTAACTTCGGGATAAG9400.2401764019019927No Hit
CTATATAACTGCGATTCGCAAGTTTTGCGGTTTTGCTAAGCCTGTTATACACTTTGGTTGGGCTGGCAACGGCCC9270.23685481336505024No Hit
GTCTAGGGAATCCGACTGTCTAATTAAAACAAAGCATTGTGATGGCCCTCGCGGGTGTTGACACAATGTGATTTC9190.234810758880778No Hit
GTATAACAGGCTTAGCAAAACCGCAAAACTTGCGAATCGCAGTTATATAGCCCAGCCCGAGTTATCTCAATCTTC8780.22433497964888255No Hit
CTCTTAACCTTGGAGACCTGCTGCGGATTCGGTACAGACTGTTGAGAATTTGCGTGGCCCTTTCATGAATTTTCA8610.21999136386980392No Hit
GCCCAGGACATCTAAGGGCATCACGGACCTGTTATTGCTCAATCTCATTTTGCTAAACACAAATTGTCCTATTAA8550.21845832300659973No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACACCCGCGAGGGCCATCACAATGCTTTGTTTTA8520.21769180257499762No Hit
ATACAATCTCGGTAACGAGAAACTTTCATGAAGAAGCCAATAGGAGGTATCGGAAGAGTTTTCTTTTCTGTTTTA8440.21564774809072537No Hit
GTACAGACTGTTGAGAATTTGCGTGGCCCTTTCATGAATTTTCAAGGTCCAATAAGAGAATATCGACACAGCAAT8070.2061939961009661No Hit
GTACATGGGAATTCCAGGTTCATGTGAACAGTTTCAGTCCACAATCCCAACTAAGAAGACGTTTTAGTGATTTCC7920.20236139394295555No Hit
CTCTTATTGGACCTTGAAAATTCATGAAAGGGCCACGCAAATTCTCAACAGTCTGTACCGAATCCGCAGCAGGTC7890.20159487351135344No Hit
CTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCGAATTTGCCGACTTCCCTTACCTACATTGATCTATCGACT7830.20006183264814925No Hit
TCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAGACCGTGCCAGTTCTGAATTGATTGTTAATTGATAA7360.1880530125530496No Hit
GTCTGTACCGAATCCGCAGCAGGTCTCCAAGGTTAAGAGTCTCTAGTCGATAGATCAATGTAGGTAAGGGAAGTC7300.18651997168984538No Hit
CTAGTCGATAGATCAATGTAGGTAAGGGAAGTCGGCAAATTCGATCCGTAACTTCGGGATAAGGATTGGCTCTGA7160.1829428763423689No Hit
AGGTAAGGGAAGTCGGCAAATTCGATCCGTAACTTCGGGATAAGGATTGGCTCTGAAGATTGAGATAACTCGGGC7130.1821763559107668No Hit
GATCAATGTAGGTAAGGGAAGTCGGCAAATTCGATCCGTAACTTCGGGATAAGGATTGGCTCTGAAGATTGAGAT7020.17936578099489242No Hit
AGTCTGTACCGAATCCGCAGCAGGTCTCCAAGGTTAAGAGTCTCTAGTCGATAGATCAATGTAGGTAAGGGAAGT6950.1775772333211542No Hit
TTCCTAAAGGGTGTGAGTTATCAATTAACAATCAATTCAGAACTGGCACGGTCTAGGGAATCCGACTGTCTAATT6920.17681071288955208No Hit
GTGTAGTTTCACCTGATAGTTTGTCTACCTCCTTGTGGGGTGACTTTATCTGCTTAATAGGACAATTTGTGTTTA6740.17221159029993943No Hit
GTATACGTTTGTATAAGGTATCTTGTATATCTATACAATCTCGGTAACGAGAAACTTTCATGAAGAAGCCAATAG6730.17195608348940541No Hit
TTCATAGAGAGATATGGTGTAATAAATTGGTAGAGCATGGCATTAAATTGCTGTGTCGATATTCTCTTATTGGAC6660.17016753581566715No Hit
GGCCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAGACCGTGCCAGTTCTGAATTGATTGTTAATT6590.16837898814192892No Hit
GGCTTAGCAAAACCGCAAAACTTGCGAATCGCAGTTATATAGCCCAGCCCGAGTTATCTCAATCTTCAGAGCCAA6570.16786797452086086No Hit
GTTATTGCTCAATCTCATTTTGCTAAACACAAATTGTCCTATTAAGCAGATAAAGTCACCCCACAAGGAGGTAGA6540.16710145408925875No Hit
ACATTGATCTATCGACTAGAGACTCTTAACCTTGGAGACCTGCTGCGGATTCGGTACAGACTGTTGAGAATTTGC6520.1665904404681907No Hit
TACAAAGACCGAAAGGGAATCCGGTTACAATTCCGGAACCTGTTGAGTATACGTTTGTATAAGGTATCTTGTATA6220.15892523615216964No Hit
GGTAAGGGAAGTCGGCAAATTCGATCCGTAACTTCGGGATAAGGATTGGCTCTGAAGATTGAGATAACTCGGGCT6120.1563701680468293No Hit
CTTGTATATCTATACAATCTCGGTAACGAGAAACTTTCATGAAGAAGCCAATAGGAGGTATCGGAAGAGTTTTCT6100.1558591544257612No Hit
ATATTAAATAGATACCTATCAGCTGTTGAGATGCTTGATACCTATTAGTCGGGTGAGTGGTTCGCCATGTATCCT6100.1558591544257612No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6090.1556036476152272No Hit
CCCTTACCTACATTGATCTATCGACTAGAGACTCTTAACCTTGGAGACCTGCTGCGGATTCGGTACAGACTGTTG6090.1556036476152272No Hit
ATCACATTGTGTCAACACCCGCGAGGGCCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAGACCGT6050.15458162037309103No Hit
TCCTTATCCCGAAGTTACGGATCGAATTTGCCGACTTCCCTTACCTACATTGATCTATCGACTAGAGACTCTTAA5910.15100452502561457No Hit
CTTATTGGACCTTGAAAATTCATGAAAGGGCCACGCAAATTCTCAACAGTCTGTACCGAATCCGCAGCAGGTCTC5870.1499824977834784No Hit
GTTGTACTCACCATGGAAGTCTTTCATAGAGAGATATGGTGTAATAAATTGGTAGAGCATGGCATTAAATTGCTG5810.1484494569202742No Hit
GTACATGGGCAGAAATCACATTGTGTCAACACCCGCGAGGGCCATCACAATGCTTTGTTTTAATTAGACAGTCGG5720.1461498956254679No Hit
ATTCTCAACAGTCTGTACCGAATCCGCAGCAGGTCTCCAAGGTTAAGAGTCTCTAGTCGATAGATCAATGTAGGT5710.14589438881493386No Hit
GCTCTGAAGATTGAGATAACTCGGGCTGGGCTATATAACTGCGATTCGCAAGTTTTGCGGTTTTGCTAAGCCTGT5680.14512786838333175No Hit
GGATTGGCTCTGAAGATTGAGATAACTCGGGCTGGGCTATATAACTGCGATTCGCAAGTTTTGCGGTTTTGCTAA5680.14512786838333175No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACACCCGCGAGGGCCATCACAATGCTTTGTTTTAA5670.1448723615727977No Hit
GTCTCTAGTCGATAGATCAATGTAGGTAAGGGAAGTCGGCAAATTCGATCCGTAACTTCGGGATAAGGATTGGCT5670.1448723615727977No Hit
ACTAGAGACTCTTAACCTTGGAGACCTGCTGCGGATTCGGTACAGACTGTTGAGAATTTGCGTGGCCCTTTCATG5660.14461685476226369No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACACCCGCGAGGGCCATCACAATGCTTTGTTTTAATTAGA5660.14461685476226369No Hit
GTACCGAATCCGCAGCAGGTCTCCAAGGTTAAGAGTCTCTAGTCGATAGATCAATGTAGGTAAGGGAAGTCGGCA5660.14461685476226369No Hit
CACCATGGAAGTCTTTCATAGAGAGATATGGTGTAATAAATTGGTAGAGCATGGCATTAAATTGCTGTGTCGATA5590.14282830708852545No Hit
GGATACACGTTGATGTCTTCATTGTAGCGCGCGTGCAGCCCAGGACATCTAAGGGCATCACGGACCTGTTATTGC5520.1410397594147872No Hit
ATCTTGTATATCTATACAATCTCGGTAACGAGAAACTTTCATGAAGAAGCCAATAGGAGGTATCGGAAGAGTTTT5480.14001773217265104No Hit
CTCACACCCTTTAGGAAGGGCCGTTGCCAGCCCAACCAAAGTGTATAACAGGCTTAGCAAAACCGCAAAACTTGC5480.14001773217265104No Hit
ATCGACTAGAGACTCTTAACCTTGGAGACCTGCTGCGGATTCGGTACAGACTGTTGAGAATTTGCGTGGCCCTTT5460.139506718551583No Hit
GGATAAGGATTGGCTCTGAAGATTGAGATAACTCGGGCTGGGCTATATAACTGCGATTCGCAAGTTTTGCGGTTT5440.1389957049305149No Hit
GTATCAACGCAGAGTACATGGGAATTCCAGGTTCATGTGAACAGTTTCAGTCCACAATCCCAACTAAGAAGACGT5440.1389957049305149No Hit
TCATTGTAGCGCGCGTGCAGCCCAGGACATCTAAGGGCATCACGGACCTGTTATTGCTCAATCTCATTTTGCTAA5430.1387401981199809No Hit
TTATTACACCATATCTCTCTATGAAAGACTTCCATGGTGAGTACAACTGTAAAACAGAAAAGAAAACTCTTCCGA5420.13848469130944685No Hit
ATTTAATGCCATGCTCTACCAATTTATTACACCATATCTCTCTATGAAAGACTTCCATGGTGAGTACAACTGTAA5380.13746266406731072No Hit
TATATAGCCCAGCCCGAGTTATCTCAATCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCGAATTTGCCGAC5360.13695165044624263No Hit
GTACATGGGATATACAAAGACCGAAAGGGAATCCGGTTACAATTCCGGAACCTGTTGAGTATACGTTTGTATAAG5360.13695165044624263No Hit
AAATCACATTGTGTCAACACCCGCGAGGGCCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAGACC5300.13541860958303842No Hit
GTTCTGAATTGATTGTTAATTGATAACTCACACCCTTTAGGAAGGGCCGTTGCCAGCCCAACCAAAGTGTATAAC5260.1343965823409023No Hit
GTAATAAATTGGTAGAGCATGGCATTAAATTGCTGTGTCGATATTCTCTTATTGGACCTTGAAAATTCATGAAAG5080.12979745975128967No Hit
CTTTCATAGAGAGATATGGTGTAATAAATTGGTAGAGCATGGCATTAAATTGCTGTGTCGATATTCTCTTATTGG4990.12749789845648335No Hit
TCCCTTACCTACATTGATCTATCGACTAGAGACTCTTAACCTTGGAGACCTGCTGCGGATTCGGTACAGACTGTT4980.12724239164594933No Hit
GTCGATATTCTCTTATTGGACCTTGAAAATTCATGAAAGGGCCACGCAAATTCTCAACAGTCTGTACCGAATCCG4980.12724239164594933No Hit
CCATGGAAGTCTTTCATAGAGAGATATGGTGTAATAAATTGGTAGAGCATGGCATTAAATTGCTGTGTCGATATT4960.12673137802488127No Hit
GAGTACAACTGTAAAACAGAAAAGAAAACTCTTCCGATACCTCCTATTGGCTTCTTCATGAAAGTTTCTCGTTAC4870.12443181673007493No Hit
TCCATGGTGAGTACAACTGTAAAACAGAAAAGAAAACTCTTCCGATACCTCCTATTGGCTTCTTCATGAAAGTTT4830.1234097894879388No Hit
GTCCTGGGCTGCACGCGCGCTACAATGAAGACATCAACGTGTATCCTATACCGAAAGGTACGGGAAATCACTAAA4790.12238776224580267No Hit
GATATACAAAGACCGAAAGGGAATCCGGTTACAATTCCGGAACCTGTTGAGTATACGTTTGTATAAGGTATCTTG4620.11804414646672406No Hit
CTCCAAGGTTAAGAGTCTCTAGTCGATAGATCAATGTAGGTAAGGGAAGTCGGCAAATTCGATCCGTAACTTCGG4580.11702211922458793No Hit
GTTTCAGTCCACAATCCCAACTAAGAAGACGTTTTAGTGATTTCCCGTACCTTTCGGTATAGGATACACGTTGAT4510.11523357155084968No Hit
ATAACAGGCTTAGCAAAACCGCAAAACTTGCGAATCGCAGTTATATAGCCCAGCCCGAGTTATCTCAATCTTCAG4480.1144670511192476No Hit
GTTGAGAATTTGCGTGGCCCTTTCATGAATTTTCAAGGTCCAATAAGAGAATATCGACACAGCAATTTAATGCCA4450.11370053068764548No Hit
ATTTAATATGATTGAGTCACGTTCGTTATCGGAATTAACCAGACAAATCATTCCACGAACTAAGAACGGCCATGC4360.11140096939283918No Hit
GAGAGATATGGTGTAATAAATTGGTAGAGCATGGCATTAAATTGCTGTGTCGATATTCTCTTATTGGACCTTGAA4310.11012343534016898No Hit
GAGATATGGTGTAATAAATTGGTAGAGCATGGCATTAAATTGCTGTGTCGATATTCTCTTATTGGACCTTGAAAA4280.10935691490856689No Hit
CTATGAAAGACTTCCATGGTGAGTACAACTGTAAAACAGAAAAGAAAACTCTTCCGATACCTCCTATTGGCTTCT4230.10807938085589672No Hit
CCTATCAGCTGTTGAGATGCTTGATACCTATTAGTCGGGTGAGTGGTTCGCCATGTATCCTTGCTGGTAGGTGTA4220.10782387404536269No Hit
CCTAAAGGGTGTGAGTTATCAATTAACAATCAATTCAGAACTGGCACGGTCTAGGGAATCCGACTGTCTAATTAA4210.10756836723482864No Hit
ATCCTATACCGAAAGGTACGGGAAATCACTAAAACGTCTTCTTAGTTGGGATTGTGGACTGAAACTGTTCACATG4200.10731286042429461No Hit
CGTTTGTATAAGGTATCTTGTATATCTATACAATCTCGGTAACGAGAAACTTTCATGAAGAAGCCAATAGGAGGT4190.10705735361376056No Hit
CTCTATGAAAGACTTCCATGGTGAGTACAACTGTAAAACAGAAAAGAAAACTCTTCCGATACCTCCTATTGGCTT4190.10705735361376056No Hit
GATATGGTGTAATAAATTGGTAGAGCATGGCATTAAATTGCTGTGTCGATATTCTCTTATTGGACCTTGAAAATT4110.1050132991294883No Hit
GTGCATGGCCGTTCTTAGTTCGTGGAATGATTTGTCTGGTTAATTCCGATAACGAACGTGACTCAATCATATTAA4060.10373576507681811No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4000.1022027242136139No Hit
CAGTTGTACTCACCATGGAAGTCTTTCATAGAGAGATATGGTGTAATAAATTGGTAGAGCATGGCATTAAATTGC4000.1022027242136139No Hit
GTCCAATAAGAGAATATCGACACAGCAATTTAATGCCATGCTCTACCAATTTATTACACCATATCTCTCTATGAA3970.10143620378201182No Hit
TCCTAAAGGGTGTGAGTTATCAATTAACAATCAATTCAGAACTGGCACGGTCTAGGGAATCCGACTGTCTAATTA3950.10092519016094373No Hit
ACCTTGGAGACCTGCTGCGGATTCGGTACAGACTGTTGAGAATTTGCGTGGCCCTTTCATGAATTTTCAAGGTCC3940.1006696833504097No Hit
TAATTAGACAGTCGGATTCCCTAGACCGTGCCAGTTCTGAATTGATTGTTAATTGATAACTCACACCCTTTAGGA3920.10015866972934165No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)