FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002146589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002146589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences391379
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTAGACCGTGCCAGTTCTGAATTGATTGTTAATTGATAACTCACACCCTTTAGGAAGGGCCGTTGCCAGCCC35620.9101152591222319No Hit
CCCTAGACCGTGCCAGTTCTGAATTGATTGTTAATTGATAACTCACACCCTTTAGGAAGGGCCGTTGCCAGCCCA23080.5897097187125523No Hit
ATGTAGGTAAGGGAAGTCGGCAAATTCGATCCGTAACTTCGGGATAAGGATTGGCTCTGAAGATTGAGATAACTC21000.536564302121473No Hit
CCCTTTAGGAAGGGCCGTTGCCAGCCCAACCAAAGTGTATAACAGGCTTAGCAAAACCGCAAAACTTGCGAATCG19390.4954277056254935No Hit
CTTTAGGAAGGGCCGTTGCCAGCCCAACCAAAGTGTATAACAGGCTTAGCAAAACCGCAAAACTTGCGAATCGCA17000.4343615779078591No Hit
TATCTATACAATCTCGGTAACGAGAAACTTTCATGAAGAAGCCAATAGGAGGTATCGGAAGAGTTTTCTTTTCTG16160.4128990058230002No Hit
GTGTATAACAGGCTTAGCAAAACCGCAAAACTTGCGAATCGCAGTTATATAGCCCAGCCCGAGTTATCTCAATCT16000.4088108968544556No Hit
ATATAGCCCAGCCCGAGTTATCTCAATCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCGAATTTGCCGACT15780.4031897470227069No Hit
TACCTACATTGATCTATCGACTAGAGACTCTTAACCTTGGAGACCTGCTGCGGATTCGGTACAGACTGTTGAGAA14150.36154213690565923No Hit
GTTATATAGCCCAGCCCGAGTTATCTCAATCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCGAATTTGCCG12680.32398263575715613No Hit
GGGCTATATAACTGCGATTCGCAAGTTTTGCGGTTTTGCTAAGCCTGTTATACACTTTGGTTGGGCTGGCAACGG12270.31350685652526067No Hit
ATATAACTGCGATTCGCAAGTTTTGCGGTTTTGCTAAGCCTGTTATACACTTTGGTTGGGCTGGCAACGGCCCTT11780.300987022809093No Hit
CAGTTATATAGCCCAGCCCGAGTTATCTCAATCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCGAATTTGC11440.2922997912509358No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10480.26777113743966846No Hit
GTATAAGGTATCTTGTATATCTATACAATCTCGGTAACGAGAAACTTTCATGAAGAAGCCAATAGGAGGTATCGG10370.2649605625237941No Hit
CTATATAACTGCGATTCGCAAGTTTTGCGGTTTTGCTAAGCCTGTTATACACTTTGGTTGGGCTGGCAACGGCCC9630.24605305854427548No Hit
GCATTAAATTGCTGTGTCGATATTCTCTTATTGGACCTTGAAAATTCATGAAAGGGCCACGCAAATTCTCAACAG9610.24554204492320741No Hit
GTGTCAACACCCGCGAGGGCCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAGACCGTGCCAGTTC9600.24528653811267337No Hit
GGTTAAGAGTCTCTAGTCGATAGATCAATGTAGGTAAGGGAAGTCGGCAAATTCGATCCGTAACTTCGGGATAAG9580.24477552449160533No Hit
GTTATACACTTTGGTTGGGCTGGCAACGGCCCTTCCTAAAGGGTGTGAGTTATCAATTAACAATCAATTCAGAAC9360.23915437465985653No Hit
GTATAGGATACACGTTGATGTCTTCATTGTAGCGCGCGTGCAGCCCAGGACATCTAAGGGCATCACGGACCTGTT8860.2263790341331548No Hit
GTGTAATAAATTGGTAGAGCATGGCATTAAATTGCTGTGTCGATATTCTCTTATTGGACCTTGAAAATTCATGAA8640.22075788430140605No Hit
GTCTAGGGAATCCGACTGTCTAATTAAAACAAAGCATTGTGATGGCCCTCGCGGGTGTTGACACAATGTGATTTC8290.21181514593271483No Hit
CTCTTAACCTTGGAGACCTGCTGCGGATTCGGTACAGACTGTTGAGAATTTGCGTGGCCCTTTCATGAATTTTCA8080.2064495029115001No Hit
GTACATGGGAATTCCAGGTTCATGTGAACAGTTTCAGTCCACAATCCCAACTAAGAAGACGTTTTAGTGATTTCC7990.20414994161669378No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACACCCGCGAGGGCCATCACAATGCTTTGTTTTA7980.20389443480615976No Hit
GTATAACAGGCTTAGCAAAACCGCAAAACTTGCGAATCGCAGTTATATAGCCCAGCCCGAGTTATCTCAATCTTC7920.20236139394295555No Hit
GTACAGACTGTTGAGAATTTGCGTGGCCCTTTCATGAATTTTCAAGGTCCAATAAGAGAATATCGACACAGCAAT7760.198273284974411No Hit
GCCCAGGACATCTAAGGGCATCACGGACCTGTTATTGCTCAATCTCATTTTGCTAAACACAAATTGTCCTATTAA7720.19725125773227487No Hit
CTCTTATTGGACCTTGAAAATTCATGAAAGGGCCACGCAAATTCTCAACAGTCTGTACCGAATCCGCAGCAGGTC7600.19418517600586643No Hit
ATACAATCTCGGTAACGAGAAACTTTCATGAAGAAGCCAATAGGAGGTATCGGAAGAGTTTTCTTTTCTGTTTTA7540.19265213514266222No Hit
TCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAGACCGTGCCAGTTCTGAATTGATTGTTAATTGATAA7390.1888195329846517No Hit
TTCCTAAAGGGTGTGAGTTATCAATTAACAATCAATTCAGAACTGGCACGGTCTAGGGAATCCGACTGTCTAATT7160.1829428763423689No Hit
GATCAATGTAGGTAAGGGAAGTCGGCAAATTCGATCCGTAACTTCGGGATAAGGATTGGCTCTGAAGATTGAGAT7120.18192084910023276No Hit
CTAGTCGATAGATCAATGTAGGTAAGGGAAGTCGGCAAATTCGATCCGTAACTTCGGGATAAGGATTGGCTCTGA6760.17272260392100752No Hit
ATATTAAATAGATACCTATCAGCTGTTGAGATGCTTGATACCTATTAGTCGGGTGAGTGGTTCGCCATGTATCCT6650.16991202900513314No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6650.16991202900513314No Hit
GTCTGTACCGAATCCGCAGCAGGTCTCCAAGGTTAAGAGTCTCTAGTCGATAGATCAATGTAGGTAAGGGAAGTC6550.16735696089979277No Hit
CTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCGAATTTGCCGACTTCCCTTACCTACATTGATCTATCGACT6500.1660794268471226No Hit
GGCCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAGACCGTGCCAGTTCTGAATTGATTGTTAATT6340.16199131787857807No Hit
ACATTGATCTATCGACTAGAGACTCTTAACCTTGGAGACCTGCTGCGGATTCGGTACAGACTGTTGAGAATTTGC6240.1594362497732377No Hit
TTCATAGAGAGATATGGTGTAATAAATTGGTAGAGCATGGCATTAAATTGCTGTGTCGATATTCTCTTATTGGAC6190.15815871572056753No Hit
GTATACGTTTGTATAAGGTATCTTGTATATCTATACAATCTCGGTAACGAGAAACTTTCATGAAGAAGCCAATAG6110.15611466123629525No Hit
AGGTAAGGGAAGTCGGCAAATTCGATCCGTAACTTCGGGATAAGGATTGGCTCTGAAGATTGAGATAACTCGGGC6060.15483712718362508No Hit
GTGTAGTTTCACCTGATAGTTTGTCTACCTCCTTGTGGGGTGACTTTATCTGCTTAATAGGACAATTTGTGTTTA6040.154326113562557No Hit
AGTCTGTACCGAATCCGCAGCAGGTCTCCAAGGTTAAGAGTCTCTAGTCGATAGATCAATGTAGGTAAGGGAAGT5860.14972699097294437No Hit
GTTGTACTCACCATGGAAGTCTTTCATAGAGAGATATGGTGTAATAAATTGGTAGAGCATGGCATTAAATTGCTG5830.1489604705413423No Hit
GGTAAGGGAAGTCGGCAAATTCGATCCGTAACTTCGGGATAAGGATTGGCTCTGAAGATTGAGATAACTCGGGCT5800.14819395010974018No Hit
GGCTTAGCAAAACCGCAAAACTTGCGAATCGCAGTTATATAGCCCAGCCCGAGTTATCTCAATCTTCAGAGCCAA5760.14717192286760403No Hit
GGATACACGTTGATGTCTTCATTGTAGCGCGCGTGCAGCCCAGGACATCTAAGGGCATCACGGACCTGTTATTGC5700.14563888200439984No Hit
GTTATTGCTCAATCTCATTTTGCTAAACACAAATTGTCCTATTAAGCAGATAAAGTCACCCCACAAGGAGGTAGA5680.14512786838333175No Hit
CTTATTGGACCTTGAAAATTCATGAAAGGGCCACGCAAATTCTCAACAGTCTGTACCGAATCCGCAGCAGGTCTC5660.14461685476226369No Hit
CTTGTATATCTATACAATCTCGGTAACGAGAAACTTTCATGAAGAAGCCAATAGGAGGTATCGGAAGAGTTTTCT5630.14385033433066158No Hit
TACAAAGACCGAAAGGGAATCCGGTTACAATTCCGGAACCTGTTGAGTATACGTTTGTATAAGGTATCTTGTATA5620.14359482752012753No Hit
GTACATGGGCAGAAATCACATTGTGTCAACACCCGCGAGGGCCATCACAATGCTTTGTTTTAATTAGACAGTCGG5490.14027323898318508No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACACCCGCGAGGGCCATCACAATGCTTTGTTTTAATTAGA5480.14001773217265104No Hit
TCCTTATCCCGAAGTTACGGATCGAATTTGCCGACTTCCCTTACCTACATTGATCTATCGACTAGAGACTCTTAA5410.13822918449891283No Hit
GTCTCTAGTCGATAGATCAATGTAGGTAAGGGAAGTCGGCAAATTCGATCCGTAACTTCGGGATAAGGATTGGCT5360.13695165044624263No Hit
ATTCTCAACAGTCTGTACCGAATCCGCAGCAGGTCTCCAAGGTTAAGAGTCTCTAGTCGATAGATCAATGTAGGT5240.13388556871983423No Hit
ATTTAATGCCATGCTCTACCAATTTATTACACCATATCTCTCTATGAAAGACTTCCATGGTGAGTACAACTGTAA5230.1336300619093002No Hit
ATCACATTGTGTCAACACCCGCGAGGGCCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAGACCGT5210.13311904828823212No Hit
ATCTTGTATATCTATACAATCTCGGTAACGAGAAACTTTCATGAAGAAGCCAATAGGAGGTATCGGAAGAGTTTT5190.13260803466716406No Hit
ATCGACTAGAGACTCTTAACCTTGGAGACCTGCTGCGGATTCGGTACAGACTGTTGAGAATTTGCGTGGCCCTTT5170.132097021046096No Hit
CCCTTACCTACATTGATCTATCGACTAGAGACTCTTAACCTTGGAGACCTGCTGCGGATTCGGTACAGACTGTTG5150.1315860074250279No Hit
TCATTGTAGCGCGCGTGCAGCCCAGGACATCTAAGGGCATCACGGACCTGTTATTGCTCAATCTCATTTTGCTAA5120.1308194869934258No Hit
TCCCTTACCTACATTGATCTATCGACTAGAGACTCTTAACCTTGGAGACCTGCTGCGGATTCGGTACAGACTGTT5110.13056398018289175No Hit
GCTCTGAAGATTGAGATAACTCGGGCTGGGCTATATAACTGCGATTCGCAAGTTTTGCGGTTTTGCTAAGCCTGT5090.13005296656182372No Hit
GGATAAGGATTGGCTCTGAAGATTGAGATAACTCGGGCTGGGCTATATAACTGCGATTCGCAAGTTTTGCGGTTT5090.13005296656182372No Hit
TATATAGCCCAGCCCGAGTTATCTCAATCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCGAATTTGCCGAC5070.12954195294075563No Hit
GTACATGGGATATACAAAGACCGAAAGGGAATCCGGTTACAATTCCGGAACCTGTTGAGTATACGTTTGTATAAG5050.12903093931968757No Hit
TTATTACACCATATCTCTCTATGAAAGACTTCCATGGTGAGTACAACTGTAAAACAGAAAAGAAAACTCTTCCGA5040.12877543250915355No Hit
CTCACACCCTTTAGGAAGGGCCGTTGCCAGCCCAACCAAAGTGTATAACAGGCTTAGCAAAACCGCAAAACTTGC5020.12826441888808546No Hit
CACCATGGAAGTCTTTCATAGAGAGATATGGTGTAATAAATTGGTAGAGCATGGCATTAAATTGCTGTGTCGATA4980.12724239164594933No Hit
GTACCGAATCCGCAGCAGGTCTCCAAGGTTAAGAGTCTCTAGTCGATAGATCAATGTAGGTAAGGGAAGTCGGCA4950.12647587121434722No Hit
AAATCACATTGTGTCAACACCCGCGAGGGCCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAGACC4940.12622036440381318No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACACCCGCGAGGGCCATCACAATGCTTTGTTTTAA4940.12622036440381318No Hit
GTATCAACGCAGAGTACATGGGAATTCCAGGTTCATGTGAACAGTTTCAGTCCACAATCCCAACTAAGAAGACGT4930.12596485759327913No Hit
GGATTGGCTCTGAAGATTGAGATAACTCGGGCTGGGCTATATAACTGCGATTCGCAAGTTTTGCGGTTTTGCTAA4890.12494283035114301No Hit
GTTCTGAATTGATTGTTAATTGATAACTCACACCCTTTAGGAAGGGCCGTTGCCAGCCCAACCAAAGTGTATAAC4880.12468732354060898No Hit
GTCGATATTCTCTTATTGGACCTTGAAAATTCATGAAAGGGCCACGCAAATTCTCAACAGTCTGTACCGAATCCG4790.12238776224580267No Hit
TCCATGGTGAGTACAACTGTAAAACAGAAAAGAAAACTCTTCCGATACCTCCTATTGGCTTCTTCATGAAAGTTT4770.12187674862473459No Hit
GTAATAAATTGGTAGAGCATGGCATTAAATTGCTGTGTCGATATTCTCTTATTGGACCTTGAAAATTCATGAAAG4710.12034370776153039No Hit
GAGTACAACTGTAAAACAGAAAAGAAAACTCTTCCGATACCTCCTATTGGCTTCTTCATGAAAGTTTCTCGTTAC4700.12008820095099634No Hit
CTCCAAGGTTAAGAGTCTCTAGTCGATAGATCAATGTAGGTAAGGGAAGTCGGCAAATTCGATCCGTAACTTCGG4620.11804414646672406No Hit
GTTGAGAATTTGCGTGGCCCTTTCATGAATTTTCAAGGTCCAATAAGAGAATATCGACACAGCAATTTAATGCCA4480.1144670511192476No Hit
ACTAGAGACTCTTAACCTTGGAGACCTGCTGCGGATTCGGTACAGACTGTTGAGAATTTGCGTGGCCCTTTCATG4470.11421154430871355No Hit
CCTAAAGGGTGTGAGTTATCAATTAACAATCAATTCAGAACTGGCACGGTCTAGGGAATCCGACTGTCTAATTAA4420.11293401025604338No Hit
CTTTCATAGAGAGATATGGTGTAATAAATTGGTAGAGCATGGCATTAAATTGCTGTGTCGATATTCTCTTATTGG4310.11012343534016898No Hit
GAGATATGGTGTAATAAATTGGTAGAGCATGGCATTAAATTGCTGTGTCGATATTCTCTTATTGGACCTTGAAAA4240.10833488766643076No Hit
GTCCTGGGCTGCACGCGCGCTACAATGAAGACATCAACGTGTATCCTATACCGAAAGGTACGGGAAATCACTAAA4230.10807938085589672No Hit
ATAACAGGCTTAGCAAAACCGCAAAACTTGCGAATCGCAGTTATATAGCCCAGCCCGAGTTATCTCAATCTTCAG4190.10705735361376056No Hit
CCATGGAAGTCTTTCATAGAGAGATATGGTGTAATAAATTGGTAGAGCATGGCATTAAATTGCTGTGTCGATATT4170.10654633999269252No Hit
GTGCATGGCCGTTCTTAGTTCGTGGAATGATTTGTCTGGTTAATTCCGATAACGAACGTGACTCAATCATATTAA4150.10603532637162444No Hit
CCTATCAGCTGTTGAGATGCTTGATACCTATTAGTCGGGTGAGTGGTTCGCCATGTATCCTTGCTGGTAGGTGTA4150.10603532637162444No Hit
GATATGGTGTAATAAATTGGTAGAGCATGGCATTAAATTGCTGTGTCGATATTCTCTTATTGGACCTTGAAAATT4130.10552431275055636No Hit
GTTTCAGTCCACAATCCCAACTAAGAAGACGTTTTAGTGATTTCCCGTACCTTTCGGTATAGGATACACGTTGAT4110.1050132991294883No Hit
TCCTAAAGGGTGTGAGTTATCAATTAACAATCAATTCAGAACTGGCACGGTCTAGGGAATCCGACTGTCTAATTA4050.1034802582662841No Hit
CGTTTGTATAAGGTATCTTGTATATCTATACAATCTCGGTAACGAGAAACTTTCATGAAGAAGCCAATAGGAGGT4040.10322475145575005No Hit
GAGAGATATGGTGTAATAAATTGGTAGAGCATGGCATTAAATTGCTGTGTCGATATTCTCTTATTGGACCTTGAA3980.10169171059254585No Hit
CTCTATGAAAGACTTCCATGGTGAGTACAACTGTAAAACAGAAAAGAAAACTCTTCCGATACCTCCTATTGGCTT3980.10169171059254585No Hit
TAATTAGACAGTCGGATTCCCTAGACCGTGCCAGTTCTGAATTGATTGTTAATTGATAACTCACACCCTTTAGGA3960.10118069697147777No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)