FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002146610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002146610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences529001
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG66081.2491469770378505Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG59431.1234383299842534Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCAACCTGCTCCCAGGGCTTCTGCTCAAGGCCCAACGCACCCAA36530.6905468987771289No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT32510.6145546038665333Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28940.547068909132497No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC23480.4438554936569118Clontech Universal Primer Mix Long (96% over 26bp)
CTCTACTTGTGCGCTATCGGTCTCTGGCCAATATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATT23000.43478178680191537No Hit
GTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCTCCACCAGGTGCTGGAAGG22290.4213602620788997No Hit
ATCCTAAGCCATAGGGAAACTCCGTTTTAAAGTGCGCTCTCGGGCGCCGCCTGGCGAAAGGGAAACCGGTTAATA20920.3954623904302638No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG20090.37977243899349905Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA19660.371643909935898Clontech Universal Primer Mix Long (96% over 26bp)
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTCGCGTGA19520.3689974121031907No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG19230.36351538087829705Clontech Universal Primer Mix Long (96% over 26bp)
CCTTTGTGCTGTCCGGTGCGCTCTCGACGACCCTTGAAAATCCGCCGGAAGGAATGATTTTCACGCGAGGTCGTA19070.3604908119266315No Hit
GTCTATCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTTAATGGCAGGTAGAGCAGCACACCTTTGTGCTGTCC17480.33043415796945563No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC17220.3255192334229992Clontech Universal Primer Mix Long (96% over 26bp)
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTCGCGTGAAAATCATTCCTTCCGGCGG16830.31814684660331455No Hit
GCACAAAGGTGTGCTGCTCTACCTGCCATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGATAGACAGTTA16650.31474420653269086No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTT16010.30264593072602886No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTGCGTTG15930.3011336462501961No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15200.2873340504082223No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT14700.2778822724342676Clontech Universal Primer Mix Long (96% over 26bp)
GTGCCAACCTGCTCCCAGGGCTTCTGCTCAAGGCCCAACGCACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGT13830.26143617875958647No Hit
GTGATAGACAGTTAAGAAGAAAAGAGAACTCTTCCCGGGGCCCCCGCCGACGTCTCCACCTTCAGTTGCGTTGCC13220.24990500963136178No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG13170.24895983183396628Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG13100.24763658291761265Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC13090.24744754735813354Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCTCC12960.24499008508490533No Hit
GTCCGGTGCGCTCTCGACGACCCTTGAAAATCCGCCGGAAGGAATGATTTTCACGCGAGGTCGTACTCATAACCG12670.23950805386001162No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT12620.23856287606261614No Hit
GAGTACGACCTCGCGTGAAAATCATTCCTTCCGGCGGATTTTCAAGGGTCGTCGAGAGCGCACCGGACAGCACAA12160.22986724032657782No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCTCCACCAGG12010.22703170693439143No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11990.22665363581543327No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAAC11370.21493343112772945No Hit
GTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCTCCACCAGGTGCTGGAAGGCGCCGGCCGTG11240.2124759688545012No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC11180.21134175549762665Clontech Universal Primer Mix Long (96% over 26bp)
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC11120.2102075421407521No Hit
GTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTACTACCAATACCTCGCCG10730.20283515532106747No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAG10700.20226804864263018No Hit
GTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTCGCGTGAAAATCATTCC10540.19924347969096468No Hit
CTACCAATACCTCGCCGCTAGGGTAGAAACGATGCCCTAGCGAGTAGGCAGGCGTGGAGGCCCGTGACGAAGCCT10300.1947066262634664No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC10080.19054784395492635Clontech Universal Primer Mix Long (96% over 26bp)
GGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCTCTAGTAACG10070.19035880839544725No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGC10040.18979170171701No Hit
CAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAAAAGCACGTGAAATTGTTAAAAGGGAAG9940.18790134612221907No Hit
GCTTAGGATCGACTAACCCGTGTCCAACTGCTGTTCACACGGAACCTTTCCCCACTTCAGTCCTCAAAGTTCTCA9780.18487677717055354No Hit
GCTCTACCTGCCATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGATAGACAGTTAAGAAGAAAAGAGAAC9610.18166317265940896No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTACTACCAA9570.1809070304214926No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC9430.17826053258878527Clontech Universal Primer Mix Long (96% over 26bp)
GCCTTGGGGGTGACCCCGGGTAGAACGGCCTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAAC9100.1720223591259752No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTA9060.17126621688805882No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCG9060.17126621688805882No Hit
CATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATGA8960.1693758612932679No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCA8930.1688087546148306No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG8860.16748550569847695Clontech Universal Primer Mix Long (96% over 25bp)
ATCCTGATGTCTTCGGATGGATTTGAGTAAGAGCATAGCTGTTGGGACCCGAAAGATGGTGAACTATGCCTGAAT8830.16691839902003966No Hit
TTCCAGCACCTGGTGGAGGACGCTCTTGGCAGGGTTCGCCCGTCCGGCGCACGCTTAACAACCAACTTAGAACTG8530.16124733223566687No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAG8500.16068022555722958No Hit
GTATTGGTAGTACGCTTGAGCGCCATCCATTTTCAGGGCTAGTTCATTCGGCAGGTGAGTTGTTACACACTCCTT8360.15803372772452226No Hit
GGTTAGTCGATCCTAAGCCATAGGGAAACTCCGTTTTAAAGTGCGCTCTCGGGCGCCGCCTGGCGAAAGGGAAAC8280.15652144324868952No Hit
GTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTACTACCAATACCTCGC8200.15500915877285676No Hit
ACCAATACCTCGCCGCTAGGGTAGAAACGATGCCCTAGCGAGTAGGCAGGCGTGGAGGCCCGTGACGAAGCCTTG8130.15368590985650313No Hit
GCACCGGACAGCACAAAGGTGTGCTGCTCTACCTGCCATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGA8110.15330783873754492No Hit
CTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTAGATGAACTAACACCTTTTGTGGTGTCTGAT8010.15141748314275397No Hit
TGCCAAGAGCGTCCTCCACCAGGTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCAACCTGCTCCCAGGGCTTCT7880.14896002086952576No Hit
CCTTGGAGACCTGCTGCGGTTATGAGTACGACCTCGCGTGAAAATCATTCCTTCCGGCGGATTTTCAAGGGTCGT7830.1480148430721303No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC7780.14706966527473483Clontech Universal Primer Mix Long (96% over 26bp)
AAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGT7630.1442341318825484No Hit
ACCCCGGGTAGAACGGCCTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAACTTTGAGGACTGA7490.14158763404984112No Hit
CTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAACTTTGAGGACTGAAGTGGGGAAAGGTTCCG7390.13969727845505017No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT7360.1391301717766129Clontech SMART CDS Primer II A (96% over 26bp)
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTT7000.13232489163536554No Hit
CCCCCAAGGCTTCGTCACGGGCCTCCACGCCTGCCTACTCGCTAGGGCATCGTTTCTACCCTAGCGGCGAGGTAT6920.13081260715953277No Hit
CCCTTATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTCGC6830.12911128712422093No Hit
ACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTCGCGTGAAAATC6800.12854418044578364No Hit
GGGTAGAAACGATGCCCTAGCGAGTAGGCAGGCGTGGAGGCCCGTGACGAAGCCTTGGGGGTGACCCCGGGTAGA6740.1274099670889091No Hit
TCCTCCACCAGGTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCAACCTGCTCCCAGGGCTTCTGCTCAAGGCCC6700.12665382485099272No Hit
ACCTTAACCTATTCTCAAACTTTAAATATGTAAGAAGTCCTTGTTACTTAGTTGAACGTGGACATTTGAATGTAT6690.1264647892915136No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6500.12287311366141085No Hit
ACCCGGGGTCACCCCCAAGGCTTCGTCACGGGCCTCCACGCCTGCCTACTCGCTAGGGCATCGTTTCTACCCTAG6470.12230600698297357No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTT6470.12230600698297357No Hit
GTACGACCTCGCGTGAAAATCATTCCTTCCGGCGGATTTTCAAGGGTCGTCGAGAGCGCACCGGACAGCACAAAG6470.12230600698297357No Hit
CCCTAGCTCCGGACAAACCGATTTCAGGGTGATAGACAGTTAAGAAGAAAAGAGAACTCTTCCCGGGGCCCCCGC6410.12117179362609902No Hit
GGATAGCAGTAACGTTTTCAGTTTTATGAGGTAAAGCGAATGATTAGAGGCCTTGGGGTTGAAACAACCTTAACC6410.12117179362609902No Hit
CTCGGGCGCCGCCTGGCGAAAGGGAAACCGGTTAATATTCCGGTACCTCGATGTGGATTATCCGCGGCAACGCAA6370.12041565138818262No Hit
GTGCATCATCGACCGATCCTGATGTCTTCGGATGGATTTGAGTAAGAGCATAGCTGTTGGGACCCGAAAGATGGT6250.1181472246744335No Hit
ATTCAGGCATAGTTCACCATCTTTCGGGTCCCAACAGCTATGCTCTTACTCAAATCCATCCGAAGACATCAGGAT6240.11795818911495443No Hit
GTCTAGATGAACTAACACCTTTTGTGGTGTCTGATAAGCGTACATTCCGGCACCTTAACCTCGCGTTCGGTTCAT6210.11739108243651714No Hit
GTTTTAAAGTGCGCTCTCGGGCGCCGCCTGGCGAAAGGGAAACCGGTTAATATTCCGGTACCTCGATGTGGATTA6160.11644590463912166No Hit
CTTCTTAACTGTCTATCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTTAATGGCAGGTAGAGCAGCACACCTT6150.11625686907964258No Hit
GCTCTAGTAACGGCGAGTGAAGCAGCAATAGCTCAAATTTGAAATCTGGCGTCTTCGACGTCCGAGTTGTAATTT6120.1156897624012053No Hit
AGGTAGAGCAGCACACCTTTGTGCTGTCCGGTGCGCTCTCGACGACCCTTGAAAATCCGCCGGAAGGAATGATTT5970.11285422900901888No Hit
GAGCTAGGGTTTAATGGCAGGTAGAGCAGCACACCTTTGTGCTGTCCGGTGCGCTCTCGACGACCCTTGAAAATC5950.11247615789006071No Hit
AGGTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAG5860.11077483785474884No Hit
GGGTAGAACGGCCTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAACTTTGAGGACTGAAGTGG5860.11077483785474884No Hit
TCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCG5850.11058580229526976No Hit
ATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTCGCGTGAAAATCATTCCTTCCG5760.10888448225995792No Hit
ATCTAGTAGCTGGTTCCTGCCGAAGTTTCCCTCAGGATAGCAGTAACGTTTTCAGTTTTATGAGGTAAAGCGAAT5750.10869544670047884No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA5750.10869544670047884Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG5630.1064270199867297Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGATTATCCGCGGCAACGCAACTGAAGGTGGAGACGTCGGCGGGGGCCCCGGGAAGAGTTCTCTTTTCTTCTTAA5610.10604894886777153No Hit
GGTTAAGGTTGTTTCAACCCCAAGGCCTCTAATCATTCGCTTTACCTCATAAAACTGAAAACGTTACTGCTATCC5600.10585991330829242No Hit
GCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGAC5570.10529280662985513No Hit
GGACAGCACAAAGGTGTGCTGCTCTACCTGCCATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGATAGAC5540.10472569995141785No Hit
GTAGAGCAGCACACCTTTGTGCTGTCCGGTGCGCTCTCGACGACCCTTGAAAATCCGCCGGAAGGAATGATTTTC5530.10453666439193877No Hit
CCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTACTACC5430.10264630879714783No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC5400.10207920211871055Clontech Universal Primer Mix Long (96% over 26bp)
GCCATAGGGAAACTCCGTTTTAAAGTGCGCTCTCGGGCGCCGCCTGGCGAAAGGGAAACCGGTTAATATTCCGGT5360.10132305988079418No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG5340.10094498876183598Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)