FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002146636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002146636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences212996
Sequences flagged as poor quality0
Sequence length75
%GC29

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55252.593945426205187No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33541.5746774587316195No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21721.019737459858401No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15220.7145674097166145No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCAACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA11320.5314653796315424RNA PCR Primer, Index 36 (96% over 30bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8500.3990685271084903No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6650.3122124359142895No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6440.3023530958327856No Hit
GTGCTGGATGGCGCCGGCCGGTTGCCCGGCTAGTGCCTTCCTGCTCCACGAGGTTTCCCCCAAGGCCCAGCGAGC5860.27512253751244153No Hit
ATCCAGCACCGCGTGGGGACGCCCTTGGCAGGCTTCGGCCGTCCGGCGGGCGATTAACGACCAACTTAGAACTGG5720.26854964412477234No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5520.25915979642810194No Hit
GTTCTAAGTTGGTCGTTAATCGCCCGCCGGACGGCCGAAGCCTGCCAAGGGCGTCCCCACGCGGTGCTGGATGGC4560.21408852748408422No Hit
ATACACATCTCCGAGCCCACGAGACCACCAACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA3180.14929857837705873RNA PCR Primer, Index 36 (96% over 30bp)
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2480.11643411143871248No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2370.11126969520554375No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2210.10375781704820748No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT2150.10094086273920637No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)