FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002146642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002146642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414339
Sequences flagged as poor quality0
Sequence length75
%GC26

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105672.5503271475772253No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCAACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA65071.5704531796427563RNA PCR Primer, Index 36 (96% over 30bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62671.5125295953313591No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50241.2125336982519144No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28260.6820502052667019No Hit
TTATACACATCTCCGAGCCCACGAGACCACCAACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA26640.6429517858565088RNA PCR Primer, Index 38 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCACCAACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA22480.5425509063834204RNA PCR Primer, Index 36 (96% over 30bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9550.23048759590576798No Hit
TATACACATCTCCGAGCCCACGAGACCACCAACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA9510.22952220283391137RNA PCR Primer, Index 36 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACCACCAACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA7800.18825164901204086RNA PCR Primer, Index 36 (96% over 30bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7190.1735294046662274No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT6100.1472224434581345No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT5810.14022334368717404No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5040.1216395270539341Clontech SMART CDS Primer II A (100% over 21bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5040.1216395270539341No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4470.10788267577997726No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4420.10667593444015648No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTT4380.10571054136829987No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)