FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002146647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002146647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences735781
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTACTTGTGCGCTATCGGTCTCTGGCCAATATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATT93451.2700790044863892No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67470.9169848093386483No Hit
GTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCAACCTGCTCCCAGGGCTTCTGCTCAAGGCCCAACGCACCCAA42390.5761225147156559No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTGCGTTG41620.5656574442667044No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39050.5307285727682558No Hit
GCTCTAGTAACGGCGAGTGAAGCAGCAATAGCTCAAATTTGAAATCTGGCGTCTTCGACGTCCGAGTTGTAATTT38300.5205353223309653No Hit
TTCCAGCACCTGGTGGAGGACGCTCTTGGCAGGGTTCGCCCGTCCGGCGCACGCTTAACAACCAACTTAGAACTG35190.4782673105176676No Hit
TTCCCAAACAACTCGACTCGTCGAAGGAGCTACGTATAGAGCGCCTTTCCGACCGCATACGGGATTCTCACCCTC30150.4097686675790758No Hit
CTCTATGACGTCCTGTTCCAAGGAACTTAGGTCGGTGGCCACTCAGAAGCATCCTCTACAAATTACAACTCGGAC28680.38978989672198655No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27320.3713061359290332No Hit
GTTTGGGAATGCAGCTCTAAATGGGAGGTAAATTTCTTCTAAAGCTAAATATTGGCCAGAGACCGATAGCGCACA25780.3503759950311302No Hit
GTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCTCCACCAGGTGCTGGAAGG25590.34779370492034994No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTT22920.31150573336359594No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCTCC21620.29383743260562584No Hit
GGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCTCTAGTAACG21180.28785739234908214No Hit
ACTCAGAAGCATCCTCTACAAATTACAACTCGGACGTCGAAGACGCCAGATTTCAAATTTGAGCTATTGCTGCTT20620.28024643202257193No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT19770.2686940815269761No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCTCCACCAGG19310.2624422212587713No Hit
GTCATAGAGGGTGAGAATCCCGTATGCGGTCGGAAAGGCGCTCTATACGTAGCTCCTTCGACGAGTCGAGTTGTT18720.25442353091476944No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAG18150.24667666058242874No Hit
CTCAGAAGCATCCTCTACAAATTACAACTCGGACGTCGAAGACGCCAGATTTCAAATTTGAGCTATTGCTGCTTC17700.24056071032005447No Hit
CTATGACGTCCTGTTCCAAGGAACTTAGGTCGGTGGCCACTCAGAAGCATCCTCTACAAATTACAACTCGGACGT17570.23879388024425746No Hit
ATTTAGAGCTGCATTCCCAAACAACTCGACTCGTCGAAGGAGCTACGTATAGAGCGCCTTTCCGACCGCATACGG17550.23852206023259637No Hit
GTGCCAACCTGCTCCCAGGGCTTCTGCTCAAGGCCCAACGCACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGT17520.23811433021510478No Hit
GGTTGGCACTAGCCTCACGGCCGGCGCCTTCCAGCACCTGGTGGAGGACGCTCTTGGCAGGGTTCGCCCGTCCGG17440.23702705016846048No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17050.23172655994106942No Hit
CTCTAGTAACGGCGAGTGAAGCAGCAATAGCTCAAATTTGAAATCTGGCGTCTTCGACGTCCGAGTTGTAATTTG15830.21514553922974364No Hit
ATACGTAGCTCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAATGGGAGGTAAATTTCTTCTAAAGCTA15690.21324279914811609No Hit
GTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCTCCACCAGGTGCTGGAAGGCGCCGGCCGTG15260.20739866889740288No Hit
CTTCTGAGTGGCCACCGACCTAAGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTATGCGGTCGGAA14840.20169044865252025No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGC14210.19312811828519627No Hit
CTATACGTAGCTCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAATGGGAGGTAAATTTCTTCTAAAGC14200.19299220827936572No Hit
CTCTATACGTAGCTCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAATGGGAGGTAAATTTCTTCTAAA13980.19000218815109388No Hit
AAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGT13940.18945854812777171No Hit
ACGTATAGAGCGCCTTTCCGACCGCATACGGGATTCTCACCCTCTATGACGTCCTGTTCCAAGGAACTTAGGTCG13850.18823535807529684No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTA13310.18089621776044776No Hit
GTCTCTGGCCAATATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATTCCCAAACAACTCGACTCGT13270.18035257773712557No Hit
CTCTAAGGGTTGGGTGCGTTGGGCCTTGAGCAGAAGCCCTGGGAGCAGGTTGGCACTAGCCTCACGGCCGGCGCC13180.17912938768465073No Hit
ACTTAGAACTGGTACGGACAAGGGGAATCTGACTGTCTAATTAAAACATAGCATTGCGATGGCCAGAAAATGGTG12990.17654709757387047No Hit
ATATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATTCCCAAACAACTCGACTCGTCGAAGGAGCTA12720.17287752741644594No Hit
GCTCTAAGGGTTGGGTGCGTTGGGCCTTGAGCAGAAGCCCTGGGAGCAGGTTGGCACTAGCCTCACGGCCGGCGC11860.16118926691501956No Hit
CTCTTGGCAGGGTTCGCCCGTCCGGCGCACGCTTAACAACCAACTTAGAACTGGTACGGACAAGGGGAATCTGAC11590.157519696757595No Hit
CTTAACAACCAACTTAGAACTGGTACGGACAAGGGGAATCTGACTGTCTAATTAAAACATAGCATTGCGATGGCC11490.15616059669928958No Hit
TCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCTCTAGTAACGGCGAGTGAAGCAGCAATAGCTCAAATTTG11320.15385012660017042No Hit
GCTACGTATAGAGCGCCTTTCCGACCGCATACGGGATTCTCACCCTCTATGACGTCCTGTTCCAAGGAACTTAGG11280.15330648657684828No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTT11280.15330648657684828No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAG11270.15317057657101774No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCA11200.15221920653020396No Hit
GTCCTGTTCCAAGGAACTTAGGTCGGTGGCCACTCAGAAGCATCCTCTACAAATTACAACTCGGACGTCGAAGAC11130.15126783648939018No Hit
GAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCTCTAGTAACGGCGAGTGAAGCAGCAA11060.1503164664485764No Hit
GAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTG10780.1465109862853213No Hit
AGGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCTCTAGTAAC10470.14229777610457459No Hit
AGCAGCAATAGCTCAAATTTGAAATCTGGCGTCTTCGACGTCCGAGTTGTAATTTGTAGAGGATGCTTCTGAGTG10460.14216186609874404No Hit
ACTTGTGCGCTATCGGTCTCTGGCCAATATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATTCCCA10360.14080276604043868No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC10350.14066685603460813No Hit
AGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTGCGTTGGGCC10350.14066685603460813No Hit
CTAGTAACGGCGAGTGAAGCAGCAATAGCTCAAATTTGAAATCTGGCGTCTTCGACGTCCGAGTTGTAATTTGTA10170.13822047592965842No Hit
CTATCGGTCTCTGGCCAATATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATTCCCAAACAACTCG9980.13563818581887818No Hit
GTATCAACGCAGAGTACATGGGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGC9950.13523045580138654No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAAC9820.13346362572558954No Hit
ATCGGTCTCTGGCCAATATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATTCCCAAACAACTCGAC9640.13101724562063985No Hit
TCCTCCACCAGGTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCAACCTGCTCCCAGGGCTTCTGCTCAAGGCCC9630.1308813356148093No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCG9610.1306095156031482No Hit
CCCATTTAGAGCTGCATTCCCAAACAACTCGACTCGTCGAAGGAGCTACGTATAGAGCGCCTTTCCGACCGCATA9460.12857086551569014No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTT9390.12761949547487636No Hit
GCTTCTGAGTGGCCACCGACCTAAGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTATGCGGTCGGA9370.12734767546321527No Hit
ATGACGTCCTGTTCCAAGGAACTTAGGTCGGTGGCCACTCAGAAGCATCCTCTACAAATTACAACTCGGACGTCG9120.1239499253174518No Hit
TGCCAAGAGCGTCCTCCACCAGGTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCAACCTGCTCCCAGGGCTTCT9100.12367810530579072No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTCGCGTGA9080.12340628529412963No Hit
TCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCG9040.12286264527080748No Hit
CACTCTACTTGTGCGCTATCGGTCTCTGGCCAATATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCA8860.12041626516585777No Hit
GTTCCAAGGAACTTAGGTCGGTGGCCACTCAGAAGCATCCTCTACAAATTACAACTCGGACGTCGAAGACGCCAG8770.11919307511338292No Hit
GGGTTGGGTGCGTTGGGCCTTGAGCAGAAGCCCTGGGAGCAGGTTGGCACTAGCCTCACGGCCGGCGCCTTCCAG8580.11661078500260268No Hit
ACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCTCTAGTAACGGCGAGTGAAGCAGCAATA8480.11525168494429729No Hit
AGTAACGGCGAGTGAAGCAGCAATAGCTCAAATTTGAAATCTGGCGTCTTCGACGTCCGAGTTGTAATTTGTAGA8440.11470804492097512No Hit
TTCTCACCCTCTATGACGTCCTGTTCCAAGGAACTTAGGTCGGTGGCCACTCAGAAGCATCCTCTACAAATTACA8300.11280530483934759No Hit
CGTATAGAGCGCCTTTCCGACCGCATACGGGATTCTCACCCTCTATGACGTCCTGTTCCAAGGAACTTAGGTCGG8200.1114462047810422No Hit
GAACTTAGGTCGGTGGCCACTCAGAAGCATCCTCTACAAATTACAACTCGGACGTCGAAGACGCCAGATTTCAAA7960.10818436464110925No Hit
AAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTGCGTTGGGCCTTGAGCAGAAGCCCTGGG7760.10546616452449846No Hit
ATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCC7750.10533025451866791No Hit
CTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAA7670.1042429744720236No Hit
GGTACGGACAAGGGGAATCTGACTGTCTAATTAAAACATAGCATTGCGATGGCCAGAAAATGGTGTTGACGCAAT7480.10166068436124336No Hit
CCCGTATGCGGTCGGAAAGGCGCTCTATACGTAGCTCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAA7410.10070931432042958No Hit
ACGTCATAGAGGGTGAGAATCCCGTATGCGGTCGGAAAGGCGCTCTATACGTAGCTCCTTCGACGAGTCGAGTTG7400.10057340431459903No Hit
GATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTGCGTTGGGCCT7390.10043749430876851No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)