FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002146681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002146681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences787975
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGCGAGGTCCCGGCCGTGAGGCTAGAACCAAGCTGGTCCGATGGGATCTGCTCAAGGCCCAACGTGCCCAA83351.0577746755924997No Hit
CCTCCACACAGTCCTGCGAGGTCCCGGCCGTGAGGCTAGAACCAAGCTGGTCCGATGGGATCTGCTCAAGGCCCA62990.7993908436181351No Hit
ATCCTAAGGCATAGGGAAACTCCGTTTTAAAGTTCGCGCTCGCGACACCCGCCGAAAGGGAAGCCGGTTAATATT30540.38757574796154703No Hit
ATGTAGGTAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGCACGTTG27370.3473460452425521No Hit
TACCTACATTGTTCTATCAACTAGAGGCTTTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTAA26180.3322440432754846No Hit
ACCCATACCCCACCGCCACGGTAGAAACCAAGCCGTGGCGAGTAGGCAGGCGCGGAGGTCAGTGACGAAGCCTTA25290.3209492686950728No Hit
ACCCAGGATCACTCCTAAGGCTTCGTCACTGACCTCCGCGCCTGCCTACTCGCCACGGCTTGGTTTCTACCGTGG24940.3165075034106412No Hit
GTTCTAAGTTGGTTGTTAAGCGCACGCCGGACGACCGAAGCCTGCCAAGAGCGTCCTCCACACAGTCCTGCGAGG23350.29632919826136617No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTACCTACATTGTTCTATCAAC19750.25064246962149817No Hit
CTCCTAAGGCTTCGTCACTGACCTCCGCGCCTGCCTACTCGCCACGGCTTGGTTTCTACCGTGGCGGTGGGGTAT19330.2453123512801802No Hit
TCGCAGGACTGTGTGGAGGACGCTCTTGGCAGGCTTCGGTCGTCCGGCGTGCGCTTAACAACCAACTTAGAACTG18870.23947460262064152No Hit
GGCTAGCAGAGCTCAGCACCTTTGTTGGGTCCGGTGCGCTCCCAACCGTCCTTGAAAATCCGGGGGATGGAATAG18430.2338906691202132No Hit
GCTCAAGGCCCAACGTGCCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCC17420.2210730035851391No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAAAAGCCTCTAGTTGATAGAACAATGTAGGTAAGGGAAGTC16580.21041276690250324No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGCACGCCGGACGACCGAAGCCTGCCAAGAGCGTCCTCC16450.20876296836828578No Hit
GTGTGGAGGACGCTCTTGGCAGGCTTCGGTCGTCCGGCGTGCGCTTAACAACCAACTTAGAACTGGTACGGACAA15310.19429550429899425No Hit
CTCCCAACCGTCCTTGAAAATCCGGGGGATGGAATAGCTTTTACGCCAGGTCGTACTCATAACCGCAGCAGGTCT15160.19239189060566644No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTTAAGCGTGCTACCCA14990.1902344617532282No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT14910.1892192011167867No Hit
GCTCCCAACCGTCCTTGAAAATCCGGGGGATGGAATAGCTTTTACGCCAGGTCGTACTCATAACCGCAGCAGGTC14880.18883847837812112No Hit
GTCCCGGCCGTGAGGCTAGAACCAAGCTGGTCCGATGGGATCTGCTCAAGGCCCAACGTGCCCAACCCTTAGAGC14820.18807703290079No Hit
CCTTTGTTGGGTCCGGTGCGCTCCCAACCGTCCTTGAAAATCCGGGGGATGGAATAGCTTTTACGCCAGGTCGTA14660.18604651162790697No Hit
GGCTAGAACCAAGCTGGTCCGATGGGATCTGCTCAAGGCCCAACGTGCCCAACCCTTAGAGCCAATCCTTTTCCC14330.18185856150258575No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGCACGCCGGACGACCGAAGCCTGCCAAGAGCGTCCTCCACACAG13750.17449792188838478No Hit
GAACCAAGCTGGTCCGATGGGATCTGCTCAAGGCCCAACGTGCCCAACCCTTAGAGCCAATCCTTTTCCCGAAGT13520.17157904755861544No Hit
ACGTAACAGAACCCGGAGACGCGGGTGGGAGCCCTGGGAAGAGTTCTCTTTTCTTCTTGATAGTCCGTCACCCTG13330.16916780354706687No Hit
GGTATGGGTAGCACGCTTAAGCGCCATCCATTTTCAGGGCTAGTTCATTCGGCAGGTGAGTTGTTACACACTCCT13180.16726418985373903No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAG13150.16688346711507346No Hit
CCTTAGGATCGACTAACCCATGTCCAACTGCTGTTCACATGGAACCTTTCCCCACTTCAGTCCTCAAAGTTCTCA12770.16206097909197628No Hit
CATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATGA12610.16003045781909325No Hit
GATCTGCTCAAGGCCCAACGTGCCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGAC12500.15863447444398618No Hit
CTGCTAGCCATTAAACCCTATCTCCAGACAAACTGATTTCAGGGTGACGGACTATCAAGAAGAAAAGAGAACTCT12290.1559694152733272No Hit
GTTGGGAGCGCACCGGACCCAACAAAGGTGCTGAGCTCTGCTAGCCATTAAACCCTATCTCCAGACAAACTGATT12260.15558869253466165No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGCAC12120.153811986420889No Hit
CATCAGACACCACAAAAGGTGTTAGTTCATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGA11780.14949712871601256No Hit
GCGCTTAAGCGTGCTACCCATACCCCACCGCCACGGTAGAAACCAAGCCGTGGCGAGTAGGCAGGCGCGGAGGTC11750.149116405977347No Hit
GCCCTGGGAAGAGTTCTCTTTTCTTCTTGATAGTCCGTCACCCTGAAATCAGTTTGTCTGGAGATAGGGTTTAAT11650.1478473301817951No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCA11280.14315174973825312No Hit
ACCGAAGCCTGCCAAGAGCGTCCTCCACACAGTCCTGCGAGGTCCCGGCCGTGAGGCTAGAACCAAGCTGGTCCG11050.14023287540848378No Hit
ACCTTAACCTATTCTCAAACTTTAAATATGTAAGAAGTCCTTGTTACTTAATTGAACGTGGACATTTGAATGTAC11000.13959833751070783No Hit
ATCCTGATGTTCTCGGATGGATTTGAGTAGGAGCATAGCTGTTGGGACCCGAAAGATGGTGAACTATGCCTGAAT10960.13909070719248706No Hit
GGGTTAGTCGATCCTAAGGCATAGGGAAACTCCGTTTTAAAGTTCGCGCTCGCGACACCCGCCGAAAGGGAAGCC10950.13896379961293187No Hit
CTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTAGATGAACTAACACCTTTTGTGGTGTCTGAT10790.13693327834004887No Hit
GGGTTAAGGTGCCGGAATGCACGCTCATCAGACACCACAAAAGGTGTTAGTTCATCTAGACAGCAGGACGGTGGC10660.1352834798058314No Hit
GTCTGGAGATAGGGTTTAATGGCTAGCAGAGCTCAGCACCTTTGTTGGGTCCGGTGCGCTCCCAACCGTCCTTGA10570.1341413115898347No Hit
GTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTTAAGCGTGCTACCCATACCCCACCG10370.13160315999873093No Hit
GAGTACGACCTGGCGTAAAAGCTATTCCATCCCCCGGATTTTCAAGGACGGTTGGGAGCGCACCGGACCCAACAA10270.13033408420317902No Hit
CAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGAAAAGAGAGTTAAACAGTACGTGAAATTGTTGAAAGGGAAG10240.12995336146451347No Hit
GTAGAAACCAAGCCGTGGCGAGTAGGCAGGCGCGGAGGTCAGTGACGAAGCCTTAGGAGTGATCCTGGGTAGAAC9890.12551159618008184No Hit
GGCCCAACGTGCCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTACCT9750.12373489006630922No Hit
GGATAGCAGTAACGTTTTCAGTTTTATGAGGTAAAGCGAATGATTAGAGGCCTTGGGGTTGAAACAACCTTAACC9710.12322725974808846No Hit
GTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTTAAGCGTGCTACCCATACCCCAC9600.12183127637298138No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTA9520.12081601573653987No Hit
GGTGGGAGCCCTGGGAAGAGTTCTCTTTTCTTCTTGATAGTCCGTCACCCTGAAATCAGTTTGTCTGGAGATAGG9500.12056220057742949No Hit
GCTCAGCACCTTTGTTGGGTCCGGTGCGCTCCCAACCGTCCTTGAAAATCCGGGGGATGGAATAGCTTTTACGCC9480.12030838541831912No Hit
GATGGGATCTGCTCAAGGCCCAACGTGCCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTG9250.11738951108854977No Hit
CATCAGGATCGGTCGATGGTGCATCCGAAGACTCCCACCTCCGTTCACGTTAATTACGCGTATGGGTTTGACACC9060.11497826707700118No Hit
GTTCTATCAACTAGAGGCTTTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTAAAAGCTATTCC8910.11307465338367334No Hit
CTTAAGCGCCATCCATTTTCAGGGCTAGTTCATTCGGCAGGTGAGTTGTTACACACTCCTTAGCGGATTCCGACT8850.11231320790634221No Hit
CCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTTAAGCGTGCTACC8770.1112979472699007No Hit
TGCCAAGAGCGTCCTCCACACAGTCCTGCGAGGTCCCGGCCGTGAGGCTAGAACCAAGCTGGTCCGATGGGATCT8760.11117103969034552No Hit
ATCCTGGGTAGAACGGCCTCTGGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAACTTTGAGGACTGA8760.11117103969034552No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGCACGCCGGACGACCGAAGCCTGCCAAGAG8750.11104413211079033No Hit
ACATTGTTCTATCAACTAGAGGCTTTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTAAAAGCT8570.10875979567879693No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGCACGCCGGACGACCG8550.10850598051968653No Hit
GTTCTGACGTGCAAATCGATCGTCAAATTTGGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTC8520.10812525778102096No Hit
GGCATAGGGAAACTCCGTTTTAAAGTTCGCGCTCGCGACACCCGCCGAAAGGGAAGCCGGTTAATATTCCGGCAC8430.10698308956502427No Hit
CAACAAAGGTGCTGAGCTCTGCTAGCCATTAAACCCTATCTCCAGACAAACTGATTTCAGGGTGACGGACTATCA8350.10596782892858275No Hit
GAATAGCTTTTACGCCAGGTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAAAAGCCTCTAGTTGATAGAACA8320.1055871061899172No Hit
CTTCACGGCAACGTAACAGAACCCGGAGACGCGGGTGGGAGCCCTGGGAAGAGTTCTCTTTTCTTCTTGATAGTC8290.10520638345125162No Hit
ATCTAGTAGCTGGTTCCTGCCGAAGTTTCCCTCAGGATAGCAGTAACGTTTTCAGTTTTATGAGGTAAAGCGAAT8190.10393730765569974No Hit
GTCCTCCACACAGTCCTGCGAGGTCCCGGCCGTGAGGCTAGAACCAAGCTGGTCCGATGGGATCTGCTCAAGGCC8080.10254132428059266No Hit
TGATAGAACAATGTAGGTAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTT8050.10216060154192709No Hit
GTCTAGATGAACTAACACCTTTTGTGGTGTCTGATGAGCGTGCATTCCGGCACCTTAACCCCACGTTCGGTTCAT7910.10038389542815443No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)