FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002146693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002146693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences670117
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG51950.7752377569887049Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG46010.6865965197122293Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT22410.33441921336124886Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT16850.25144862762771275No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC16280.2429426503133035Clontech Universal Primer Mix Long (96% over 26bp)
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC15430.23025829817778087No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG15010.2239907359461109Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA13750.20518804925110093Clontech Universal Primer Mix Long (96% over 26bp)
GTGAATAAGTGCACATGGTGGATGCCTTGGCGATTACAGGCGATGAAGGACGTAGTAGCTTGCGATAAGCTGCGG13200.19698052728105692No Hit
CCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACAGTCGCAGCCACCATTTTATTGCAAC12050.17981934498005572No Hit
GTTATGGCTAGGGGGTCATTGCGACTTACCAACCCATTGCAAACTCCGAATACCGATGAGTGCGAGCTTGGGAGA11860.17698401920858595No Hit
CTACTAGGGCGTACCTTATCCCGAAGTTACGGTACCAATTTGCCGAGTTCCTTCTCCCGAGTTCTCTCAAGCGCC11580.17280564438747265No Hit
AGCCATAACAGTGCTCTACCCCCGATGGTGATACTTGAGGCACTACCTAAATAGTTTTCGGAGAGAACCAGCTAT11550.17235796137092477No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG11340.16922418025508978Clontech Universal Primer Mix Long (96% over 26bp)
GTTCTAAGTTGGTTGTTCAGCGTAGCCCAGTCTTCCCCCTTGCGGGGGAAACCGTGCTCTTCCACACGCAGATCC11110.16579194379488957No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT10970.1637027563843329Clontech Universal Primer Mix Long (96% over 26bp)
GGTCTACACCCAGCGACTGTCGCCCTATTCGGACTCGATTTCTCTACGGCTCCCCTATTCGGTTAACCTTGCCAC10880.1623597073346893No Hit
CCCTAGTAGTTTGACCACTTTACTGTGGAAGGATGAACGGGTTGCAATAAAATGGTGGCTGCGACTGTTTAATAA10780.16086743061286313No Hit
GTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCTCCACCAGGTGCTGGAAGG10700.1596736092354022No Hit
TTCCAGCACCGGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACAG10590.15803210484139338No Hit
ATACATAGGTATGCAAAGCGAACGCGGCGAACTGAAACATCTAAGTAGCTGCAGGAAAAGAAATCAACCGAGATT10410.15534600674210622No Hit
GTACTGGTTCACTATCGGTCGATTACGAGTATTTAGCCTTGGAGGATGGTCCCCCCATGTTCAGACAGGATTTCT9780.14594466339460124No Hit
GTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCAACCTGCTCCCAGGGCTTCTGCTCAAGGCCCAACGCACCCAA9720.14504929736150554No Hit
TCATACGACGGTGCAGGAATATTAACCTGCTTCCCATCAGCTACGCATCTCTGCCTCGCCTTAGGGGCCGACTCA9140.13639409237491362No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC8850.13206648988161768Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCG8830.13176803453725244No Hit
ATTCTAAGGCGCTTGAGAGAACTCGGGAGAAGGAACTCGGCAAATTGGTACCGTAACTTCGGGATAAGGTACGCC8730.13027575781542627No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTGCGTTG8680.1295296194545132No Hit
ACTCTGTAGTGTCCGGTGCGTATGTGACGGCCCTTGAAAATCCGAGGGAAAGAATCAGTTTCGTGCCTGGTCGTA8680.1295296194545132No Hit
GTGTAGACCCGAAACCAAGTGATCTACCCATGGCCAGGATGAAGGTGCGGTAACACGCCCTGGAGGTCCGAACCC8650.1290819364379653No Hit
GTGTATCACAGTGCAACTAGTCTGCAGTCAAAGACGGGATTCTCACCCTCTCTGATACCCTGTTCCAAAGGATTT8270.12341128489502579No Hit
GTGCTGTGTTTTTATTAAACAGTCGCAGCCACCATTTTATTGCAACCCGTTCATCCTTCCACAGTAAAGTGGTCA8240.12296360187847793No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG8160.12176978050101699Clontech Universal Primer Mix Long (96% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC8060.12027750377919079Clontech Universal Primer Mix Long (96% over 26bp)
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTT8030.11982982076264295No Hit
GCCCAGACTACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTA7970.11893445472954724No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTAGTCTG7720.11520376292498175No Hit
GTCTTCCCCCTTGCGGGGGAAACCGTGCTCTTCCACACGCAGATCCGCTCCCGGTCCGCAACCGCCTTGCGGCAG7600.11341303085879034No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACTGGGACTTCAATCAAGAGCTTGCACCCCATCATTTAATCTT7530.112368437153512No Hit
GGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTA7410.11057770508732057No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG7360.10983156672640748Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGATGCCTTGGCGATTACAGGCGATGAAGGACGTAGTAGCTTGCGATAAGCTGCGGGGAGCTAGCAAACGAGC7360.10983156672640748No Hit
GTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTAT7350.10968233905422485No Hit
GTGCAGGAATATTAACCTGCTTCCCATCAGCTACGCATCTCTGCCTCGCCTTAGGGGCCGACTCACCCTGCTCCG7160.1068470132827551No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTT7080.10565319190529415No Hit
CCTTAGAATACTCATCTCGCCCACCTGTGTCGGTTTGCGGTACGGTCTCGTATGACTGAAGCTTAGAGGCTTTTC7040.10505628121656367No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAAC7010.10460859820001582No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6950.1037132321669201No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTCAGCGTAGCCCAGTCTTCCCCCTTGCGGGGGAAACCGTGCTC6750.10072867872326773No Hit
GTAGTGTCCGGTGCGTATGTGACGGCCCTTGAAAATCCGAGGGAAAGAATCAGTTTCGTGCCTGGTCGTACTCAT6710.10013176803453726No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)