FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002146696

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002146696
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196579
Sequences flagged as poor quality0
Sequence length75
%GC28

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43022.1884331490138824No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27421.3948590642947618No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCTGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA22271.1328778760701805TruSeq Adapter, Index 5 (96% over 29bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21931.1155820306339945No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12860.654189918556916No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6640.33777768734198466No Hit
ATACACATCTCCGAGCCCACGAGACTCGCTGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA5580.28385534568799314TruSeq Adapter, Index 5 (96% over 29bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5530.2813118390062011No Hit
TTATACACATCTCCGAGCCCACGAGACTCGCTGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA4530.23044170537036002TruSeq Adapter, Index 5 (96% over 29bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4270.21721547062504132No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3780.19228910514347922No Hit
CAGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2280.11598390468971762No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT2210.11242299533520875No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT2100.10682728063526623No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)