FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002146700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002146700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220799
Sequences flagged as poor quality0
Sequence length75
%GC27

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49202.2282709613721075No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30361.3750062273832762No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22000.9963813241907797No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCTGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA20580.932069438720284TruSeq Adapter, Index 5 (96% over 29bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14400.652176866743056No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7950.3600559785143954No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6600.29891439725723395No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5810.2631352497067469No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5560.25181273465912435No Hit
ATACACATCTCCGAGCCCACGAGACTCGCTGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA5130.23233800877721367TruSeq Adapter, Index 5 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACTCGCTGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA3560.16123261427814436TruSeq Adapter, Index 5 (96% over 29bp)
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG2670.12092446070860827No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT2430.11005484626289068No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT2320.10507293964193679No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)